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Oxygen-dependent transcriptome profiling in R.sphaeroides 2.4.1 in the dark

ABSTRACT: Wild type (2.4.1) strain, dark conditions, controlled oxtgen concentration in the growth medium (achieved by constant sparging with a gas mixture containing 1% CO2, variable - indicated for every sample - concentration O2 and balance N2). Keywords: dose response

ORGANISM(S): Cereibacter sphaeroides Cereibacter sphaeroides 2.4.1

PROVIDER: GSE7004 | GEO | 2007/02/14

SECONDARY ACCESSION(S): PRJNA99251

REPOSITORIES: GEO

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Oxygen-dependent transcriptome profiling in R.sphaeroides 2.4.1 in the dark

Project description:Wild type (2.4.1) strain, dark conditions, controlled oxtgen concentration in the growth medium (achieved by constant sparging with a gas mixture containing 1% CO2, variable - indicated for every sample - concentration O2 and balance N2). Keywords: dose response Cells were harvested at OD 0.16-0.18 after rapid addition of rifampicin, spun down and frozen for subsequent RNA isolation. RNA was isolated using bead beater and RNeasy kit. Traces of DNA were removed by DNAse I treatment until level non-detectable by 33-36 cycles of real-time PCR. Further sample preparaton -according Affymetrix protocols. Data processing: MAS 5.0 values are deposited here (for compatability with other submitted samples), values used in the publications are obtained with RMA and may be recreated using the supplied CEL files.

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