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BMP signaling differentially regulates distinct hematopoietic stem cell types

ABSTRACT: In BRE-GFP transgenic mice BMP activated cells are marked by GFP expression. Analysis of GFP+ and GFP- LSK-SLAM HSC enriched fractions from fetal liver and adult bone marrow shows the interinsic differences in the genetic program of these two HSC enriched fractions.

ORGANISM(S): Mus musculus

PROVIDER: GSE70132 | GEO | 2015/06/23

SECONDARY ACCESSION(S): PRJNA287690

REPOSITORIES: GEO

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BMP signaling differentially regulates distinct hematopoietic stem cell types

Project description:In BRE-GFP transgenic mice BMP activated cells are marked by GFP expression. Analysis of GFP+ and GFP- LSK-SLAM HSC enriched fractions from fetal liver and adult bone marrow shows the interinsic differences in the genetic program of these two HSC enriched fractions. RNAseq of GFP+ and GFP- LSK-SLAM cells from BRE-GFP transgenic mice Fetal liver and adult bone marrow

2015-06-23 | E-GEOD-70132 | biostudies-arrayexpress

AGM hematopoietic stem cells are differentially regulated by BMP and Hedgehog signalling pathways

Project description:The first HSCs are produced in the aorta-gonadmesonephros (AGM) region of the embryo through endothelial to a hematopoietic transition. BMP4 and Hedgehog affect their production/expansion, but it is unknown whether they act to affect the same HSCs. In this study using the BRE GFP reporter mouse strain that identifies BMP/Smad-activated cells, we find that the AGM harbors two types of adult-repopulating HSCs upon explant culture. Embryonic day 11 AGM are dissected and either analyzed directly, or after explant culture in conditions containing BMP/Hedgehog with or without cyclopamine. EC: endothelial enriched (CD31+Kit-); MC: mesenchymal cell enriched (CD31-Kit-); HPSC: hematopoietic progenitor/stem cell enriched; AGM11: E11 fresh AGMs; AGMex: AGM after explant culture; AGMcy: AGM after explant in presence of cyclopamine; CD31p: CD31 positive; CD31n: CD31 negative; KITp: c-Kit positive; KITn: c-Kit negative; BREp: BRE-GFP positive; BREn: BRE-GFP negative

2015-12-22 | E-GEOD-76253 | biostudies-arrayexpress

AGM hematopoietic stem cells are differentially regulated by BMP and Hedgehog signalling pathways

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Differential expression of Hdc-GFP+/hi HSC VS Hdc-GFP-/lo hematopoetic stem cells (HSCs) from bone marrow.

Project description:Bone marrow Hdc-GFPhi and Hdc-GFPlo HSPC (SLAM-LSK, Lin-c-kit+Sca-1+CD150+CD48-) HSCs were isolated from mouse femur and tibia from histidine decarboxylase (Hdc) green fluorescent protein (Hdc-GFP) mice. Hdc-GFPhi HSC and Hdc-GFPlo HSC cells were sorted by combinations of GFP and the cell surface markers of HSC. total RNA was isolated from sorted 2,500 HSPCs using ARCTURUS PicoPure RNA isolation kit (Life Technologies). cDNA was amplified and libraries were constructed by using SMARTer Ultra Low Input RNA kit (Clontech Laboratories) and Nextera XT DNA Library Preparation kit (Illumina) according to manufacturer’s instructions respectively. Sequencing was performed on the Illumina HiSeq2000 platform.

2019-11-20 | GSE90787 | GEO

Sorted HSCs from aged Specific Pathogen Free and germ free mice

Project description:To begin to explore mechanisms by which microbiota signals regulate HSC lineage bias, gene expression profiling was performed on sorted LSK-SLAM cells from aged SPF and aged GF mice.

2021-11-10 | GSE183138 | GEO

Small RNA-Seq of LSK Slam HSCs MxCre Control vs MxCre Meg3 flox (Meg3 KO)

Project description:Here, we present a Small RNA-Seq dataset of isolated mouse hematopoietic stem cells (HSC LSK slam; Lineage- Sca-1+ c-Kit+ CD150+CD48-) of Meg3 KO (induced MxCre Meg3mat flox/pat wt) and control (induced MxCre) cells

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Project description:We have performed RNA-seq in highly purified Hematopoeitic Stem cells (HSC, LSK/SLAM) from young (4-5mo) and old (20-24mo) C57BL/6J in order to investigate differences in gene expression between these groups.

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A comprehensive transcriptome signature of murine hematopoietic stem cell aging

Project description:We have performed ATAC-seq in highly purified Hematopoeitic Stem cells (HSC, LSK/SLAM) from young (4-5mo) and old (20-24mo) C57BL/6J in order to investigate differences in chromatin accessible sites between these 2 groups.

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Continuous mitotic activity of primitive hematopoietic stem cells in adult mice

Project description:Transcriptomes of 282 single HSC (LSK CD48-lo/CD150+) isolated from bone marrow of un-induced Col1a1 H2B-GFP xR26rtTA mice

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RNA-Seq to identify germline stem cell-enriched genes and cystoblast-enriched genes and BMP targeted genes

Project description:We sequenced the transcriptomes of genetically expanded stem cells that either experience high levels of BMP signalling (expression of constitutively active BMPR, tkvQD.) or low levels (expression of shRNA again the differentiation promoting factor bam). We also sequenced RNA from differentiating daughter cells (cystoblasts) isolated through the expression of bam.GFP. We then performed a differential expression analysis. Our aim was to identify germline stem cell-enriched gene and cystoblast-enriched genes by tkvQD vs bam-GFP. We also wished to identify possible BMP target genes by comparing tkvQD and bam.shRNA. We have subsequently taken a few of the genes identified and validated their roles in germline stem cell self-renewal and differentiation in vivo.

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