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Expression data for Fat storage inducing transmembrane protein 2 (FIT2/FITM2)-deleted small intestine

ABSTRACT: Tamoxifen- induced FIT2 deletion using whole body inducible ROSA26CreERT2 FIT2 floxed mice causes upregulation of the p53 pathway which accompanies intestinal death We used microarrays to detail the changes in small intestine gene expression at day 2, 3, and 5 of tamoxifen induced FIT2 deletion in whole body inducible ROSA26CreERT2 FIT2 floxed mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE70465 | GEO | 2015/07/03

SECONDARY ACCESSION(S): PRJNA288752

REPOSITORIES: GEO

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Expression data for Fat storage inducing transmembrane protein 2 (FIT2/FITM2)-deleted small intestine

Project description:Tamoxifen- induced FIT2 deletion using whole body inducible ROSA26CreERT2 FIT2 floxed mice causes upregulation of the p53 pathway which accompanies intestinal death We used microarrays to detail the changes in small intestine gene expression at day 2, 3, and 5 of tamoxifen induced FIT2 deletion in whole body inducible ROSA26CreERT2 FIT2 floxed mice. Small intestine was harvested from L/L and ROSA26CreERT2 L/L mice at days 2, 3, and 5 post- tamoxifen treatment and subject to microarray analysis. Pooled intestinal RNA from n biological replicates were used for each genotype and timepoint. D2 L/L n=6, D2 KO n=7, D3 L/L n=6, D3 KO n=6, D5 L/L n=8, D5 KO n=7.

2015-07-03 | E-GEOD-70465 | biostudies-arrayexpress

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RNA sequencing of control and dnm2-knockout mouse sciatic nerves

Project description:In this study, we analyzed the transcriptome profiles of mouse sciatic nerves after developmental (P1 and P5) or inducible (4 weeks post Tamoxifen) deletion of Dnm2 conditionally in Schwann cells (using a P0Cre-driven or a P0CreERT2-driven recombination of floxed alleles, respectively) as compared to controls (floxed Dnm2 homozygous, Cre-negative).

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Sall1CreER E13.5 110228

Project description:We have employed whole genome microarray expression profiling to identify genes regulated by Sall1 in the kidney. Kidneys at E13.5 were obtained from inducible Sall1 deletion 24 hrs after tamoxifen treatment (2 set).

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Project description:In this study, we have used inducible and tissue-specific genetic deletion to investigate the function of HBO1 in the hematopoietic system. RNA-seq was used to examine the dependence of gene expression on the presence of HBO1(KAT7). Two different conditional expression sytems were used to induce Cre recombinase and delete a floxed allele of HBO1 in this study. This was to control for the treatement effect during Cre induction (interferon induction of Mx1-cre vs. tamoxifen induction of Rosa26-CreERT2). This data set indicates that HBO1 promotes expression of multiple genes encoding a hematopoietic transcription factor network essential for HSC quiescence and self-renewal in adult hematopoiesis.

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mRNA-seq data for mouse ovarian tumors arising from oviduct with combinations of (Tamoxifen-inducible) inactivation of Brca1, Trp53, Rb1, and Nf1, or Apc and Pten, and normal ovaries and oviducts.

Project description:We engineered mice to have Ovgp1 promotor-driven expression of a tamoxifen-inducible Cre recombinase (iCre-ERT2), which inactivated the function of combinations of engineered floxed alleles of combinations of tumor suppressor genes in mouse oviducts (Fallopian tube) when mice were treated with tamoxifen. 21 samples were from oviductal tumors from mice with combinations of floxed alleles of Brca1, Trp53, Rb1, and/or Nf1 (BPRN mice), of which we analyzed 19. 6 samples were from oviductal tumors of mice with floxed alleles of Apc and Pten. 4 control samples were from pooled ovaries from BPRN mice that were not tamoxifen treated, and 4 other control samples were similar pools from mouse oviducts. Our goal was to investigate the similarity of these mouse models of ovarian cancer with different subgroups of human ovarian cancer. We provide raw read data, mapped count data for genes and transcripts, several normalizations of the count data, and supplementary files that contain our first analyses, and may be convenient for consumers wanting to investigate details about their favorite genes. The supplementary files are briefly described in our supplementary file Readme_GEOsupplements_s0.txt.

2021-04-19 | GSE135590 | GEO

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