Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data for Fat storage inducing transmembrane protein 2 (FIT2/FITM2)-deleted small intestine

ABSTRACT: Tamoxifen- induced FIT2 deletion using whole body inducible ROSA26CreERT2 FIT2 floxed mice causes upregulation of the p53 pathway which accompanies intestinal death We used microarrays to detail the changes in small intestine gene expression at day 2, 3, and 5 of tamoxifen induced FIT2 deletion in whole body inducible ROSA26CreERT2 FIT2 floxed mice. Small intestine was harvested from L/L and ROSA26CreERT2 L/L mice at days 2, 3, and 5 post- tamoxifen treatment and subject to microarray analysis. Pooled intestinal RNA from n biological replicates were used for each genotype and timepoint. D2 L/L n=6, D2 KO n=7, D3 L/L n=6, D3 KO n=6, D5 L/L n=8, D5 KO n=7.

ORGANISM(S): Mus musculus

SUBMITTER: David Silver

PROVIDER: E-GEOD-70465 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

NEUROMESO

Project description:Exposure of mouse ESCs to a sequence of extrinsic signals, which recapitulates in vivo development, generates a bipotential neuromesodermal precursor (NMP) that can be directed to differentiate into either spinal cord or paraxial mesodermal tissue. To induce differentiation,mouse ES cells were plated on CellBINDSurface dishes (Corning) precoated with 0.1% gelatin (Sigma) at a density of 5x10^3 cells cm-2 in N2B27 medium. Cells were grown in N2B27 supplemented with 10ng/ml bFGF (R&D) for 3 days (D1-D3) and then were transferred into serum free media without bFGF (D3-D5). To induce ventral hindbrain identity NPCs (NH) 100nM RA (Sigma) and 500nM SAG (Calbiochem) was added from D3-D5. Spinal cord identity (NP) was induced by the addition of 5nM CHIR 99021 (Axon) from D2 to D3 followed by 100nM RA, 500nM SAG from D3-D5. To induce mesodermal differentiation the cells were treated with CHIR99021 from D2-D5.

2014-07-18 | E-MTAB-2268 | biostudies-arrayexpress

Impact of the short chain fatty acid, sodium butyrate, on gut resident microbiota, gut mucus proteome and fish susceptibility to bacterial and parasitic infections in vivo

Project description:Previous works in the framework of EU ARRAINA Project evidenced a pro-inflammatory condition in gilthead sea bream (Sparus aurata) fed extremely low fish meal/fish oil diets, and this effect was mostly reversed by butyrate supplementation. The hypothesis of work is that these nutritionally-mediated changes can be extensive to intestinal mucus proteome and gut microbiota, which in turn could modify disease outcome.s If so, the prevalence and progression of the disease might be also modified by diet composition and feed additives. Gilthead sea bream fingerlings were fed with control and experimental diets formulated by BioMar until two year-old. FM was added at 25% in the control diet (D1) and at 5% in the other three diets (D2-D4). Added oil was either FO (D1 control diet) or a blend of vegetable oils, replacing the 58% (D2) and the 84% (D3-D4 diets) of FO. A commercial sodium butyrate preparation (NOREL, BP70) was added to the D4 diet at 0.4%. At month 20, 6 fish per each dietary treatment were sampled for iTRAQ profiling and fingerprinting of intestinal mucus proteome. Mucus collected from anterior and posterior intestine segments was trypsin digested, labelled with iTRAQ reagents, isoelectrofocused and resolved by LC-MS/MS. More than 1000 proteins were unequivocally annotated and principal component analysis clearly separated anterior and posterior segments. The diet effect with changes in the abundance of approximately 120 proteins was restricted to anterior section with a reversion of the pattern of the extreme diet (D3 fish) with dietary butyrate supplementation. Butyrate supplementation also reversed the decrease of microbiotay diversity associated with D3 feeding, and led to a improvement the disease outcomes in fish challenged with Photobacterium damselae and the intestinal parasite Enteromyxum leei.

2018-10-24 | PXD006183 | Pride

RNA-seq datasets from differentiating iHA-Foxk1 and Foxk1 KO mESCs in mesodermal conditions

Project description:D3, D5 and D10 EBs from iHA-Foxk1 and Foxk1 KO cells were harvested for RNA and submitted for sequencing to understand the transcriptional profile of these cells during differentiation in the presence and absence of FOXK1

2023-05-08 | GSE202562 | GEO

ATAC-seq datasets from differentiating iHA-Foxk1 and Foxk1 KO mESCs in mesodermal conditions

Project description:50k cells from D3, D5 and D10 EBs from iHA-Foxk1 and Foxk1 KO cells were harvested for ATAC-seq and submitted for sequencing to understand the chromatin dynamics of these cells during differentiation in the presence and absence of FOXK1

2023-05-08 | GSE202517 | GEO

Effect of Arid1a deletion in antiviral effector CD8+ T cells. [RNA-seq]

Project description:To investigate the roll of ARID1A in the regulation of effector CD8+ T cell responses, we crossed GzmB-Cre P14 Thy1.1 mice with Arid1a-fl/fl mice to generate Arid1a Het and KO mice. We then performed gene expression profiling analysis of WT, Het, and KO cells in vivo at d3, d5, and d8 post-LCMV Armstrong infection.

2023-06-13 | GSE227634 | GEO

Expression data for Fat storage inducing transmembrane protein 2 (FIT2/FITM2)-deleted small intestine

Project description:Tamoxifen- induced FIT2 deletion using whole body inducible ROSA26CreERT2 FIT2 floxed mice causes upregulation of the p53 pathway which accompanies intestinal death We used microarrays to detail the changes in small intestine gene expression at day 2, 3, and 5 of tamoxifen induced FIT2 deletion in whole body inducible ROSA26CreERT2 FIT2 floxed mice.

2015-07-03 | GSE70465 | GEO

mRNA time-course transcription profiling of bacteriocytes and gut tissue of pea aphids reared on standard artificial diet (AP3) and artificial diet depleted in Tyr and Phe (YFÃ)

Project description:Tyrosine (Tyr, Y) and phenylalanine (Phe, F) synthesis is shared by the pea aphid and its symbiont Buchnera aphidicola.These aromatic amino acids are essential for the pea aphid growth and development. To characterize the molecular mechanisms, at gene transcriptional level, underlying this symbiotic integrated network pea aphids (Acyrthosyphon pisum, clone LL01) were reared on (i) standard artificial diet (AP3) and (ii) on the same AP3 medium depleted of Tyr (Y) and Phe (F). From each of the two groups, aphids were collected at specific time points and dissected: 12 h (D0), 1 day (D1), 2 days (D2), 3 days (D3), 4 days (D4), 5 days (D5) and 7 days (D7). Total RNA, to be used in gene expression analysis by arrays, was extracted, under the two rearing conditions, from two tissues: gut [from 20 aphids per sample at all 7 time points] and bacteriocytes [from 25 aphids per sample at 4 time points: 3 days (D3), 4 days (D4), 5 days (D5) and 7 days (D7)]. At each time point we included three biological replicates.

2017-07-18 | E-MTAB-4456 | biostudies-arrayexpress

A clinical study to demonstrate bioequivalence between two drugs Xeloda (Reference) and Capecitabine 500 mg Tablets (Test) in cancer patients

Project description:Intervention1: Capecitabine Tablets : Dose & Frequency: 500 mg per day Duration: 3 days (D1, D3, D5) Mode of Administration: Oral Control Intervention1: Capecitabine (Xeloda) Tablets: Dose & Frequency: 500 mg per day Duration: 3 days (D1, D3, D5) Mode of Administration: Oral Primary outcome(s): Establish the bioequivalence of Test vs Reference in relation to the rate and extent of absorption on the basis of the following pharmacokinetic parameters: â?¢Cmax â?¢AUC0tTimepoint: â?¢D1, D3, D5 â?¢D1, D3, D5 Study Design: Other Method of generating randomization sequence:Computer generated randomization Method of allocation concealment:Centralized Blinding and masking:Open Label

| 2573355 | ecrin-mdr-crc

Effect of Arid1a deletion on chromatin accessibility in antiviral effector CD8+ T cells. [ATAC-Seq]

Project description:To investigate the role of ARID1A in the regulation of effector CD8+ T cell responses, we crossed GzmB-Cre P14 Thy1.1 mice with Arid1a-fl/fl mice to generate Arid1a Het and KO mice. We then performed chromatin accessibility profiling analysis by ATAC-seq in WT, Het, and KO cells in vivo at d0, 48h in vitro activated, d3, d5, and d8 post-LCMV Armstrong infection.

2023-06-13 | GSE228171 | GEO

202303_Benchmark Datasets for pepDESC

Project description:This dataset includes: Proteome Discoverer result files for D1, D2, and D3. Quantification result files for D1, D2, and D3. raw data files for D1 and D2. Raw data files for D3 are deposited via PRIDE with identifer PXD003881.

2022-10-28 | MSV000090606 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data