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Chromosomal origin utilisation in Haloferax meiditerranei wild type and mutant strains

ABSTRACT: We have comprehensively explored the origin utilisation in Haloferax mediterranei. Here we report three active chromosomal origins by genome-wide replication profiling, and demonstrate that when these three origins are deleted, a dormant origin becomes activated.

ORGANISM(S): Haloferax mediterranei

PROVIDER: GSE70597 | GEO | 2015/07/08

SECONDARY ACCESSION(S): PRJNA289136

REPOSITORIES: GEO

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Chromosomal origin utilisation in Haloferax meiditerranei wild type and mutant strains

Project description:We have comprehensively explored the origin utilisation in Haloferax mediterranei. Here we report three active chromosomal origins by genome-wide replication profiling, and demonstrate that when these three origins are deleted, a dormant origin becomes activated. Genomic DNA was extracted from the H. mediterranei cultures at specific time points(12h,18h,24h,36h,42h and stationary phase 66h), or during the exponential phase (OD600≈0.5) and stationary phase (OD600≈4.0)for the origin deletion strains. After being lablled, Genomic DNA from exponential phase and stationary phase were hybridized to Haloferax mediterranei genome array genechips.

2015-07-08 | E-GEOD-70597 | biostudies-arrayexpress

Checking the origin utilisation of Haloferax mediterranei H13 through high-throughput DNA sequencing

Project description:A potential origin that appears to stay dormant in its native host Haloferax volcanii lacking the main active origins becomes activated and competent for replication of the entire chromosome when integrated into the chromosome of the origin-deleted H. mediterranei. Measurement of replication dynamics (marker frequency analysis; MFA) for Haloferax mediterranei H13

2015-07-10 | E-GEOD-70592 | biostudies-arrayexpress

Checking the origin utilisation of Haloferax mediterranei H13 through high-throughput DNA sequencing

2015-07-10 | GSE70592 | GEO

Haloferax mediterranei

Project description:Checking the origin utilisation of Haloferax mediterranei H13 through high-throughput DNA sequencing

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Genes differentially expressed in Haloferax mediterranei strains, DF50 and M-NM-^TdeoR2, with or without induction by fructose

Project description:Transcriptional profiling of Haloferax mediterranei DF50 and M-NM-^TdeoR2 with induction by fructose comparing with the strains without this induction. Goal was to explore the effect of induction by fructose on Haloferax mediterranei. Total RNA from the Haloferax mediterranei DF50 and M-NM-^TdeoR2 with or without induction by fructose were used to generate target cDNA, and then hybridized to Haloferax mediterranei genome array genechips, representing about 3800 genes.

2013-12-18 | E-GEOD-41134 | biostudies-arrayexpress

Genes differentially expressed Haloferax mediterranei strains: control wild-type strain vs M-NM-^TphaA1 strain

Project description:Transcriptional profiling of Haloferax mediterranei comparing control wild-type strain with M-NM-^TphaA1 strain, in which phaA1 gene are knockouted. M-NM-^TphaA1 strain can accumulate PHB only. Goal was to explore the PHA biosynthetic pathway and to determine their impact on primary metabolism in H. mediterranei. Total RNA from the control Haloferax mediterranei and its M-NM-^TphaA1 strain were used to generate target cDNA, and then hybridized to 8*15K Haloferax mediterranei genome array genechips, representing about 3800 genes.

2012-04-19 | E-GEOD-37420 | biostudies-arrayexpress

Genes differentially expressed Haloferax mediterranei strains: control wild-type strain vs M-NM-^TphaEC strain

Project description:Transcriptional profiling of Haloferax mediterranei comparing control wild-type strain with M-NM-^TphaEC strain, in which PHA synthase genes are knockouted. M-NM-^TphaEC strain is deficient in PHBV accumulation. Goal was to explore the PHBV biosynthetic pathway and to determine their impact on primary metabolism in H. mediterranei. Total RNA from the control Haloferax mediterranei and its M-NM-^TphaEC strain were used to generate target cDNA, and then hybridized to 8*15K Haloferax mediterranei genome array genechips, representing about 3800 genes.

2012-04-19 | E-GEOD-37421 | biostudies-arrayexpress

Genes differentially expressed in Haloferax mediterranei strains, DF50 and ΔdeoR2, with or without induction by fructose

Project description:Transcriptional profiling of Haloferax mediterranei DF50 and ΔdeoR2 with induction by fructose comparing with the strains without this induction. Goal was to explore the effect of induction by fructose on Haloferax mediterranei.

2013-12-18 | GSE41134 | GEO

Haloferax mediterranei

Project description:Chromosomal origin utilisation in Haloferax meiditerranei wild type and mutant strains

| PRJNA289136 | ENA

Genes differentially expressed Haloferax mediterranei strains: control wild-type strain vs ΔphaA1 strain

Project description:Transcriptional profiling of Haloferax mediterranei comparing control wild-type strain with ΔphaA1 strain, in which phaA1 gene are knockouted. ΔphaA1 strain can accumulate PHB only. Goal was to explore the PHA biosynthetic pathway and to determine their impact on primary metabolism in H. mediterranei.

2012-04-20 | GSE37420 | GEO

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