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Transcriptomic profiling of S. cerevisiae wild type and aft2 mutant in response to exposure to selenite

ABSTRACT: The yeast S. cerevisiae does not require selenium for growth, so it is an excellent model to investigate the toxicity of this element without interference from its requirement as growth factor as occurs in animal cells. Exposure to selenite interferes with the yeast iron metabolism. Aft2 is a transcription factor related (with Aft1) to maintenance of iron homeostasis. We have found that aft2 cells are highly sensitive to selenite (a common environmental source for selenium). In these experiments we investigate the transcriptional response to a sub-lethal dose of selenite in wild type and aft2 cells. Our results show that the aft2 mutation strongly potentiates the transcriptional response to selenite, showing induction of many genes related to the responses to oxidative stress and DNA damage.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE70835 | GEO | 2015/12/31

SECONDARY ACCESSION(S): PRJNA289663

REPOSITORIES: GEO

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Transcriptomic profiling of S. cerevisiae wild type and aft2 mutant in response to exposure to selenite

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