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Cbx8 acts non-canonically with Wdr5 to promote mammary tumorigenesis [RNA-Seq]

ABSTRACT: Here we show that in MMTV-Myc cells (1) MS culture enriches for aggressive breast cancer cells compared to the bulk cells, (2) Cbx8 knockdown reduces Notch-network gene expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE71076 | GEO | 2016/06/21

SECONDARY ACCESSION(S): PRJNA290315

REPOSITORIES: GEO

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Cbx8 acts non-canonically with Wdr5 to promote mammary tumorigenesis [ChIP-Seq]

Project description:Here we found that Cbx8 knockdown in MMTV-Myc and MDA-MB-231-Luc cells reduces H3K4me3 mark on Notch-network genes

2016-06-21 | GSE71003 | GEO

Cbx8 Acts Non-canonically with Wdr5 to Promote Mammary Tumorigenesis.

Project description:Chromatin-mediated processes influence the development and progression of breast cancer. Using murine mammary carcinoma-derived tumorspheres as a functional readout for an aggressive breast cancer phenotype, we performed a loss-of-function screen targeting 60 epigenetic regulators. We identified the Polycomb protein Cbx8 as a key regulator of mammary carcinoma both in vitro and in vivo. Accordingly, Cbx8 is overexpressed in human breast cancer and correlates with poor survival. Our genomic analyses revealed that Cbx8 positively regulates Notch signaling by maintaining H3K4me3 levels on Notch-network gene promoters. Ectopic expression of Notch1 partially rescues tumorsphere formation in Cbx8-depleted cells. We find that Cbx8 associates with non-PRC1 complexes containing the H3K4 methyltransferase complex component WDR5, which together regulate Notch gene expression. Thus, our study implicates a key non-canonical role for Cbx8 in promoting breast tumorigenesis.

| S-EPMC4972459 | biostudies-literature

Cbx8 acts non-canonically with Wdr5 to promote mammary tumorigenesis

Project description:Cbx8 acts non-canonically with Wdr5 to promote mammary tumorigenesis

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Cbx8 acts non-canonically with Wdr5 to promote mammary tumorigenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-06-21 | GSE71077 | GEO

Cbx8 acts non-canonically with Wdr5 to promote mammary tumorigenesis [ChIP-Seq]

Project description:Cbx8 acts non-canonically with Wdr5 to promote mammary tumorigenesis [ChIP-Seq]

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CBX8 KO and WT re-expression RNA-seq

Project description:T98G CBX8 KO and WT re-expression RNA-seq analysis to identify potential CBX8 gene targets

2018-12-13 | GSE123689 | GEO

REPROGRAMMING CBX8-PRC1 FUNCTION WITH A POSITIVE ALLOSTERIC MODULATOR

Project description:Canonical targeting of Polycomb Repressive Complex 1 (PRC1) to repress developmental genes is mediated by cell type-specific, paralogous chromobox (CBX) proteins (CBX2, 4, 6, 7 and 8). Based on their central role in silencing and their dysregulation associated with human disease including cancer, CBX proteins are attractive targets for small molecule chemical probe development. Here, we have used a quantitative and target-specific cellular assay to discover a potent positive allosteric modulator (PAM) of CBX8. The PAM activity of UNC7040 antagonizes H3K27me3 binding by CBX8 while increasing interactions with nucleic acids. We show that treatment with UNC7040 leads to efficient and selective eviction of CBX8-containing PRC1 from chromatin, loss of silencing and reduced proliferation across different cancer cell lines. Our discovery and characterization of UNC7040 not only reveals the most cellularly potent CBX8-specific chemical probe to date, but also corroborates a mechanism of Polycomb regulation by non-specific CBX nucleotide binding activity.

2022-01-03 | GSE182592 | GEO

CBX8 promotes LUAD growth and metastasis through transcriptional repression of CDKN2C and SCEL

Project description:CBX8 promotes LUAD cell proliferation and migration through downregulating the expression of CDKN2C and SCEL.The mRNAs from A549 cells expressing control shRNAs or CBX8 shRNAs were extracted and subjected to RNA-seq.

2023-06-16 | GSE234537 | GEO

CBX8 together with SET facilitates ovarian carcinoma growth and metastasis by suppressing the transcription of SUSD2

Project description:CBX8 promotes cell proliferation and migration through downregulating the expression of SUSD2.The mRNAs from SKOV3 cells expressing control shRNAs or CBX8 shRNAs were extracted and subjected to RNA-seq.

2021-12-21 | GSE191191 | GEO

A cDNA microarray analysis was performed on shRNA KDs of EZH2, SUZ12, BMI1, or CBX8 HepG2 cells.

Project description:Polycomb group (PcG) proteins including EZH2, SUZ12 ,BMI1,CBX8 and so on, which specifically catalyze trimethylation of histone 3 lysine 27 (H3K27me3), and methylated H3K27 can be recognized by other specific binding proteins to compress chromatin structure, leading to the transcriptional repression of the target genes. To explore a potential functional implication of PcG components in HCC, we stably transfected HepG2 cells with either vectors or constructs expressing shRNA that specifically targets EZH2, SUZ12, BMI1, or CBX8. A cDNA microarray analysis was performed on shRNA KDs of EZH2, SUZ12, BMI1, or CBX8 HepG2 cells. To obtain a broader understanding of the molecular network of PcG in HCC, the whole genome microarray expression profiling was performed on shRNA KDs of EZH2, SUZ12, BMI1, or CBX8 HepG2 cells. Comparison of gene expression results from shRNA KDs of EZH2, SUZ12, BMI1 or CBX8 HepG2 cells.

2014-01-16 | E-GEOD-54108 | biostudies-arrayexpress

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