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Effects of biobanking on chromatin accessibility

ABSTRACT: The goal of this study was to test the effects of biobanking on chromatin assessibility, as defined by ATAC-seq. Human naïve B cells were FACS sorted from PBMCs that were either frozen or processed fresh. A range of cell numbers were used to identify technical limitations and reproducibility of the ATAC-seq assay on biobanked PBMCs comapred to freshly sorted material. To provide insight into a disease process, naive B cells were isolated from healthy donors or a biorepository of Systemic Lupus Erythematosus undergoing disease flares. These data identify a unique signature of accessible chromatin in SLE B cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE71338 | GEO | 2016/05/22

SECONDARY ACCESSION(S): PRJNA290920

REPOSITORIES: GEO

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