Methylation profiling

Dataset Information

0

Comparison of the DNA methylation profiles of CD14+ monocytes from human peripheral blood with DCs and MACs obtained by exposure with GM-CSF/IL-4 and GM-CSF, respectively, and with mature DCs and MACs after LPS exposure


ABSTRACT: Comparison of the DNA methylation profiles of CD14+ monocytes from human peripheral blood with derived dendritic cells (DCs) and macrophages (MACs) obtained by exposure with GM-CSF/IL-4 and GM-CSF, respectively, and with mature DCs and MACs after lipopolysaccharide (LPS) exposure The methylation profiles of bisulfite-modified DNA of human CD14+ monocytes were compared with derived immature dendritic cells (iDCs) and macrophages (iMACs) following GM-CSF/IL-4 and GM-CSF incubation, and then activation/maturation with lypopolysaccharyde (LPS) using the Infinium HumanMethylation450 BeadChips (Illumina, Inc., San Diego, CA,). This platform allows the interrogation of >485,000 methylation sites per sample at single-nucleotide resolution, and comprises an average of 17 CpG sites per gene in the 99% of RefSeq genes. 96% of CpG islands are covered, with additional coverage in CpG island shores and the regions flanking them. The samples were hybridized in the array following the manufacturer’s instructions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE71837 | GEO | 2015/12/11

SECONDARY ACCESSION(S): PRJNA292302

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-12-11 | E-GEOD-71837 | biostudies-arrayexpress
2015-12-12 | E-GEOD-75937 | biostudies-arrayexpress
2015-04-21 | E-GEOD-68061 | biostudies-arrayexpress
2015-12-12 | GSE75937 | GEO
2017-09-11 | GSE103210 | GEO
2017-08-15 | GSE90831 | GEO
2015-12-12 | E-GEOD-75938 | biostudies-arrayexpress
2023-02-23 | GSE210935 | GEO
2015-12-12 | GSE75938 | GEO
2019-05-09 | GSE130881 | GEO