Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CnAb1 knock down in R1 mESCs differentiated to mesoderm in Ebs

ABSTRACT: R1 mESCs were trasnfected with siRNAs against CnAb1 or Control (Luciferase) using Lipofectamine. mRNA was extracted 48 hours using Qiagen RNeasy Mini kit using DNAse treatment Microarray was performed after QPCR validation of the downregulation of CnAb1.

ORGANISM(S): Mus musculus

PROVIDER: GSE72102 | GEO | 2018/08/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

CnAb1 knock down in R1 mESCs culture in pluripotent conditions

Project description:R1 mESCs were trasnfected with siRNAs against CnAb1 or Control (Luciferase) using Lipofectamine. mRNA was extracted 48 hours using Qiagen RNAeasy Mini kit Microarray was performed after QPCR validation of the downregulation of CnAb1.

2018-08-14 | GSE72101 | GEO

Effect of BMP4 and noggin on gene expression in murine R1 ES cells

2010-03-01 | GSE20527 | GEO

Effect of BMP4 and noggin on gene expression in murine R1 ES cells

Project description:To confirm that the SMAD1/5- and SMAD4-associated genes are direct transcriptional regulators in mESCs in response to BMP, we treated undifferentiated R1 ES cells with BMP4 or with the BMP4 antagonist noggin, which can inhibit BMP signaling effectively for 4 h. Undifferentiated R1 ES cells were treated for 4 h with BMP4 or with the BMP4 antagonist noggin, which can inhibit BMP signaling effectively. Untreated R1 ES cells served as the control.

2010-03-07 | E-GEOD-20527 | biostudies-arrayexpress

R1 ESC

Project description:R1

2011-05-07 | GSE29102 | GEO

Mouse embryonic stem cells (mESCs) and neurons overexpressing DNMT1 and DNMT1 Y495C mutant form

Project description:Genome wide DNA methylation profiling of mESCs and neurons overexpressing DNMT1 and DNMT1 Y495C mutant form. The Infinium Mouse methylation Beadchip was used to obtain DNA methylation profiles across approximately > 285k methylation sites in DNA samples of mESCs and neurons. Samples included wild-type cell line (R1) and transgenic cell lines (R1wtDnmt1 and R1Dnmt1Y495C).

2023-12-01 | GSE241544 | GEO

Comparison of R1, R2 and R3

Project description:3 samples of R1, R2 and R3 bone marrow monocytes were compared from 3 biological replicates in 3 separate experiments. R1, R2 and R3 were sorted from triplicate experiments from pools of mice

2016-11-24 | GSE90471 | GEO

Expression data of mESCs differentiated into Paraxial mesoderm

Project description:Stem cell-derived tissues have wide potential for modelling developmental and pathological processes as well as cell-based therapy. However, it has proven difficult to generate several key cell types in vitro, including skeletal muscle. In vertebrates, skeletal muscles derive during embryogenesis from the presomitic mesoderm (PSM). Using PSM development as a guide, we establish conditions for the differentiation of monolayer cultures of mouse embryonic stem (ES) cells into PSM-like cells without the introduction of transgenes or cell sorting. We differentiated mouse ESCs in serum-free medium supplemented with Rspo3 ( or as an alternative with Chir 9902) and the Bmp inhibitor LDN193189. In vivo, the PSM cells are first expressing Msgn1 (posterior PSM marker) and then mature to express Pax3 (anterior PSM marker). After 4 days of differentiation of mESCs, Msgn1-positive cells were FACS-sorted and their transcriptome analyzed. After 6 days of differentiation, Pax3-positive cells were sorted and their transcriptome analyzed. Mouse ESCs differentiated for 0, 4 and 6 days in serum-free medium containing a Wnt activator, a BMP inhibitor and DMSO, to study paraxial mesoderm in vitro

2015-08-03 | E-GEOD-71348 | biostudies-arrayexpress

Laser-capture microdissection and RNA sequencing of aleurone and starchy endosperm of maize R1 genotypes

Project description:Three homozygous R1 genotypes, R1-g (WT), R1-sc and r1-m3 were laser microdissected and sequenced. The data showed that in aleurone (AL), expression of R1 in R1-sc and r1-m3 is higher than R1-g, and R1 is ectopically expressed in starchy endosperm (SE) in R1-sc and r1-m3. Interestingly, the ectopic expression of R1 in SE may be associated with RNA splicing process.

2022-07-21 | GSE200905 | GEO

Expression data of mESCs differentiated into Paraxial mesoderm

2015-08-03 | GSE71348 | GEO

Mouse R1 ES cell SAGE library

Project description:Mouse R1 ES cell SAGE library Keywords: other

2005-09-01 | GSE3233 | GEO