Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Study of RFX1 and RFX3 binding sites in the mouse genome

ABSTRACT: The aim of the study was to identify RFX1 and RFX2 binding sites in mouse pancreatic beta cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE72272 | GEO | 2015/08/22

SECONDARY ACCESSION(S): PRJNA293562

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Study of RFX1 and RFX3 binding sites in the mouse genome

Project description:The aim of the study was to identify RFX1 and RFX2 binding sites in mouse pancreatic beta cells. Chromatin immunoprecipitation experiments were performed with the mouse MIN6 beta cell line and antibodies raised against RFX1 and RFX3. Immunoprecipitated DNA was sequenced using the Genome Analyzer II (Illumina). Antibodies were described in Reith W, Ucla C, Barras E, Gaud A, Durand B, Herrero-Sanchez C, Kobr M, Mach B : RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins. Mol Cell Biol 1994;14:1230–1244

2015-08-22 | E-GEOD-72272 | biostudies-arrayexpress

Next-Generation Sequencing Analysis of Wild Type and Rfx1-/- or Rfx2-/- or Rfx3-/- Mouse Ependymal Cells

Project description:Purpose: This study was carried out to determine the consequences of inactivating either Rfx1, or Rfx2 or Rfx3 on gene expression during differentiation of mouse multiciliated ependymal cells.

2020-07-13 | GSE145323 | GEO

Next-Generation Sequencing Analysis of ChIP

Project description:Purpose: This study was carried out to determine RFX1 or RFX2 or RFX3 binding sites in nuclei of multiciliated ependymal cells. Methods: DNA was extracted from ependymal cell cultures. ChIP-sequencing was used to determine the binding sites of RFX1 , RFX2 or RFX3.

2020-07-13 | GSE144758 | GEO

Effect of RFX1 on gene expression and the potential binding target genes in LPS-stimulated macrophages [RNA-seq]

Project description:To investigate the function of RFX1 in the regulation of macrophage polarization, we overexpressed RFX1 in mouse peritoneal macrophages(PMAs) or human CD14+ derived macrophage(hMDMs) by lentivirus infections or reduce RFX1 expression in PMAs from mice with Rfx1 deficiency(CKO) . We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points.

2023-11-08 | GSE210667 | GEO

ChIP-seq for RFX1

Project description:ChIP-seq for RFX1

| PRJNA866861 | ENA

RNA-seq Analysis of RFX1-dependent Genes in Fusarium graminearum Z-3639

Project description:Fusarium graminearum is a major pathogen of Fusarium head blight in wheat, barley, and rice, as well as ear rot and stalk rot in maize. Regulatory Factor X (RFX) transcription factors are well-conserved in animals and fungi, but their functions are diverse, ranging from DNA-damage response to ciliary gene regulation. We investigated the role of the sole RFX transcription factor, RFX1, in F. graminearum. Deletion of rfx1 resulted in multiple defects in hyphal growth, conidiation, virulence, and sexual development. Deletion mutants of rfx1 were more sensitive to various types of DNA damage than the wild-type strain. Septum formation was inhibited and micronuclei were produced in the rfx1 deletion mutants. The results of the neutral comet assay demonstrated that disruption of rfx1 function caused spontaneous DNA double-strand breaks. To understand regulatory mechanisms of rfx1 in F. graminearum, we obtained and analyzed genome-wide transcription profiles generated from the RNA-sequencing data of the wild-type and M-NM-^Trfx1 strains. RNA-sequencing-based transcriptomic analysis revealed that RFX1 suppressed the expression of many genes, including genes for the repair of DNA damage. 2 samples examined: mycelia harvested 24 h after inoculation of wild-type conidia in complete medium; mycelia harvested 32 h after inoculation of M-NM-^Trfx1 conidia in complete medium

2014-01-28 | E-GEOD-52198 | biostudies-arrayexpress

RNA-seq Analysis of RFX1-dependent Genes in Fusarium graminearum Z-3639

Project description:Fusarium graminearum is a major pathogen of Fusarium head blight in wheat, barley, and rice, as well as ear rot and stalk rot in maize. Regulatory Factor X (RFX) transcription factors are well-conserved in animals and fungi, but their functions are diverse, ranging from DNA-damage response to ciliary gene regulation. We investigated the role of the sole RFX transcription factor, RFX1, in F. graminearum. Deletion of rfx1 resulted in multiple defects in hyphal growth, conidiation, virulence, and sexual development. Deletion mutants of rfx1 were more sensitive to various types of DNA damage than the wild-type strain. Septum formation was inhibited and micronuclei were produced in the rfx1 deletion mutants. The results of the neutral comet assay demonstrated that disruption of rfx1 function caused spontaneous DNA double-strand breaks. To understand regulatory mechanisms of rfx1 in F. graminearum, we obtained and analyzed genome-wide transcription profiles generated from the RNA-sequencing data of the wild-type and Δrfx1 strains. RNA-sequencing-based transcriptomic analysis revealed that RFX1 suppressed the expression of many genes, including genes for the repair of DNA damage.

2014-01-28 | GSE52198 | GEO

Effect of of RFX1 on regene expression in mouse peritoneal macrophages

Project description:To investigate the function of RFX1 in the regulation of macrophage polarization, we overexpressed RFX1 in mouse peritoneal macrophages(PMAs) by lentivirus infections. We then performed gene expression profiling analysis using data obtained from RNA-seq.

2024-08-06 | GSE236155 | GEO

ChIP-sequencing of Human FOXN2 and RFX1 in HEK293T Cells

Project description:We have performed ChIP-sequencing analysis on human FOXN2 and RFX1 target sequences in human embryonic kidney HEK293T cells stably expressing Streptavidin-S-FLAG (SFB) triple-tagged proteins. The NGS sequencing were performed on Illumina MiSeq desktop sequencer.

2015-01-01 | E-MTAB-3120 | biostudies-arrayexpress

Next-Generation Sequencing Analysis of Wild Type and Rfx1-/- or Rfx2-/- or Rfx3-/- Mouse Ependymal Cells

Project description:Next-Generation Sequencing Analysis of Wild Type and Rfx1-/- or Rfx2-/- or Rfx3-/- Mouse Ependymal Cells

| PRJNA606759 | ENA