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A transcription factor pulse can prime chromatin for heritable transcriptional memory [RNAseq]

ABSTRACT: To indentify genes displaying long-term transcriptionnal memory (LTTM) we used a trans-differentiation system where Bcell are reprogramed into macrophages upon induction of the transcription factor Cebpa. We performed RNAseq in untreated Bcells, after 12h of Cebpa induction and after 6days of “wash” of Cebpa, to see if any genes respond more/faster in a second stimulation than in the first.

ORGANISM(S): Mus musculus

PROVIDER: GSE72487 | GEO | 2016/12/21

SECONDARY ACCESSION(S): PRJNA294145

REPOSITORIES: GEO

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A transcription factor pulse can prime chromatin for heritable transcriptional memory [ChIPseq]

Project description:To investigate the epigenetic control of long-term transcriptionnal memory (LTTM) we used a trans-differentiation system where Bcell are reprogramed into macrophages upon induction of the transcription factor Cebpa. We performed ChIPseq in untreated Bcells, after 12h of Cebpa induction and after 6days of “wash” of Cebpa, to see if any proteins/histone marks are retained.

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A transcription factor pulse can prime chromatin for heritable transcriptional memory

Project description:This SuperSeries is composed of the SubSeries listed below.

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