Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dynamic changes in E protein activity orchestrate germinal center and plasma cell development

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE72832 | GEO | 2016/05/12

SECONDARY ACCESSION(S): PRJNA295174

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Dynamic changes in E protein activity orchestrate germinal center and plasma cell development

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-05-12 | E-GEOD-72832 | biostudies-arrayexpress

Dynamic changes in Id3 and E-protein activity orchestrate germinal center and plasma cell development.

Project description:The generation of high-affinity antibodies requires germinal center (GC) development and differentiation of long-lived plasma cells in a multilayered process that is tightly controlled by the activity of multiple transcription factors. Here, we reveal a new layer of complexity by demonstrating that dynamic changes in Id3 and E-protein activity govern both GC and plasma cell differentiation. We show that down-regulation of Id3 in B cells is essential for releasing E2A and E2-2, which in a redundant manner are required for antigen-induced B cell differentiation. We demonstrate that this pathway controls the expression of multiple key factors, including Blimp1, Xbp1, and CXCR4, and is therefore critical for establishing the transcriptional network that controls GC B cell and plasma cell differentiation.

| S-EPMC4886367 | biostudies-literature

Dynamic changes in E protein activity orchestrate germinal center and plasma cell development [ChIP-Seq]

Project description:Humoral immunity requires the generation of high-affinity antibodies, which involves the generation of germinal centres (GC) promoting class switch and affinity maturation of antigen-specific B cells, and the differentiation of long-lived plasma cells. This multi-layered process is tightly controlled by the activity of a transcriptional network including Bcl6, essential for the development of GC, and Blimp1, required for the differentiation of plasma cells. Here, we reveal an additional layer of complexity by demonstrating that dynamic changes in E-protein activity mediated by Id3 govern both GC and plasma cell differentiation. We show that down-regulation of Id3 expression in B cells in essential for releasing E2A and E2-2, the combined activity of which is required for both GC B cell and plasma cell differentiation. We demonstrate that this pathway controls the expression of multiple key factors required for antigen-induced B cell differentiation, including Blimp1, Xbp1, Mef2b and CXCR4 and is therefore critical for establishing the transcriptional network that controls GC B cell and plasma cell differentiation. Genome binding of transcription factor E2A

2016-05-13 | E-GEOD-72828 | biostudies-arrayexpress

Dynamic changes in E protein activity orchestrate germinal center and plasma cell development [RNA-Seq]

2016-05-13 | E-GEOD-72831 | biostudies-arrayexpress

Dynamic changes in E protein activity orchestrate germinal center and plasma cell development [RNA-Seq]

2016-05-13 | GSE72831 | GEO

Dynamic changes in E protein activity orchestrate germinal center and plasma cell development [ChIP-Seq]

2016-05-13 | GSE72828 | GEO

Dynamic changes in E protein activity orchestrate germinal center and plasma cell development

Project description:Dynamic changes in E protein activity orchestrate germinal center and plasma cell development

| PRJNA295174 | ENA

Development of plasma cell-prone germinal center B cells

Project description:Higher- or lower-affinity GC B cells are directed to plasma cell or recycling GC cell fates; however, how commitment to the plasma cell fate takes place is unclear. By using the level of Bcl6 as a marker, we found that a population of light zone (LZ) GC cells, Bcl6loCD69hi with IRF4 and higher-affinity BCRs or Bcl6hiCD69hi with lower- affinity BCRs, favored the plasma cell or recycling GC cell fate, respectively. Mechanistically, CD40 acted as a dose-dependent regulator for Bcl6loCD69hi cell formation. Furthermore, we found that ICAM1 and SLAM levels on Bcl6loCD69hi cells were higher than on Bcl6hiCD69hi cells, thereby affording more stable TFH-GC B cell contacts, while attenuating this contact down-regulated IRF4. These data support the model that commitment to the plasma cell begins in the GC, and suggest that stability and possibly duration of TFH-GC B cell contacts are key for formation of plasma cell-prone precursor GC cells.

2018-03-29 | GSE109732 | GEO

Transcriptional regulation of germinal center B and plasma cell fates by dynamical control of IRF4

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-05-04 | E-GEOD-46608 | biostudies-arrayexpress

CRTC2 couples genotoxic stress and plasma cell differentiation in the germinal center

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-01-01 | GSE23171 | GEO