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FOXO1 Regulates Uterine Epithelial Integrity and Progesterone Receptor Expression Critical for Embryo Implantation [RNA-seq]

ABSTRACT: We report the sequencing of the uterine transcriptome sequencing of control and conditional knock-out of the Forkhead box O1 at day 4.5 of murine pseudopregnancy

ORGANISM(S): Mus musculus

PROVIDER: GSE72895 | GEO | 2018/10/15

REPOSITORIES: GEO

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FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity [RNA-seq]

Project description:We report the sequencing of the uterine transcriptome sequencing of control and conditional knock-out of the Forkhead box O1 at day 4.5 of murine pseudopregnancy RNA was isolated from day 4.5 Pseudopregnancy adult females. RNA from two females was pooled per sample. Three samples per genotype were sequenced for differential gene expression analysis.

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FOXO1 regulates uterine epithelial integrity and progesterone receptor expression critical for embryo implantation.

Project description:Successful embryo implantation requires a receptive endometrium. Poor uterine receptivity can account for implantation failure in women who experience recurrent pregnancy loss or multiple rounds of unsuccessful in vitro fertilization cycles. Here, we demonstrate that the transcription factor Forkhead Box O1 (FOXO1) is a critical regulator of endometrial receptivity in vivo. Uterine ablation of Foxo1 using the progesterone receptor Cre (PgrCre) mouse model resulted in infertility due to altered epithelial cell polarity and apoptosis, preventing the embryo from penetrating the luminal epithelium. Analysis of the uterine transcriptome after Foxo1 ablation identified alterations in gene expression for transcripts involved in the activation of cell invasion, molecular transport, apoptosis, ?-catenin (CTNNB1) signaling pathway, and an increase in PGR signaling. The increase of PGR signaling was due to PGR expression being retained in the uterine epithelium during the window of receptivity. Constitutive expression of epithelial PGR during this receptive period inhibited expression of FOXO1 in the nucleus of the uterine epithelium. The reciprocal expression of PGR and FOXO1 was conserved in human endometrial samples during the proliferative and secretory phase. This demonstrates that expression of FOXO1 and the loss of PGR during the window of receptivity are interrelated and critical for embryo implantation.

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Expression profiling of the uteri of progesterone induced uterine gland knockout mice

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FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity [ChIP-seq]

Project description:We report the whole genome binding profile of the Forkhead Box O1

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FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity [ChIP-seq]

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Enhancing Uterine Receptivity for Embryo Implantation through Controlled Collagenase Intervention

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Gene expression profiling in periimplantation mouse uterine luminal epithelium

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