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Enhancing Uterine Receptivity for Embryo Implantation through Controlled Collagenase Intervention

ABSTRACT: Ineffective endometrial matrix remodeling, a key factor in infertility, impedes embryo implantation in the uterine wall. Our study reveals the cellular and molecular impact of human collagenase-1 administration in mouse uteri, demonstrating enhanced embryo implantation rates. Collagenase-1 promotes remodeling of the endometrial extracellular matrix (ECM), degrading collagen fibers and proteoglycans. This process releases matrix-bound bioactive factors, (e.g. VEGF, decorin), facilitating vascular permeability and angiogenesis. Collagenase-1 elevates embryo implantation regulators, including NK cell infiltration and the key cytokine LIF. Remarkably, uterine tissue maintains structural integrity despite reduced endometrial collagen fiber tension. In-utero collagenase-1 application rescues implantation in the heat stress and embryo transfer models, known for low implantation rates. Importantly, ex-vivo exposure of human uterine tissue to collagenase-1 induces collagen de-tensioning and VEGF release, mirroring remodeling observed in mice. Our research highlights collagenase potential to induce and orchestrate cellular and molecular processes enhancing uterine receptivity for effective embryo implantation. This innovative approach underscores ECM remodeling mechanisms critical for embryo implantation

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Uterus

SUBMITTER: Idan Adir

LAB HEAD: Irit Sagi

PROVIDER: PXD054389 | Pride | 2024-08-13

REPOSITORIES: Pride

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			Action	DRS
	Sandbox_IS_Idan_Tamar_DeCell.msf	Msf
	Sandbox_IS_Idan_Tamar_DeCell.mzML	Mzml
	Sandbox_IS_Idan_Tamar_DeCell.mzid.gz	Mzid
	Sandbox_IS_Idan_Tamar_DeCell_MMP_1.raw	Raw
	Sandbox_IS_Idan_Tamar_DeCell_MMP_2.raw	Raw

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