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Transcriptional profiling of C. elegans aptf-1(gk7954) mutant in L4 sleep stage.

ABSTRACT: To investigate which genes are up- or down-regulated during L4 sleep in C. elegans mutant aptf-1(gk794) we performed whole genome microarray expression profiling using the C. elegans (V2) Gene Expression Microarray, 4x44K from Agilent Technologies.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE73282 | GEO | 2015/09/22

SECONDARY ACCESSION(S): PRJNA296527

REPOSITORIES: GEO

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Transcriptional profiling of C. elegans aptf-1(gk7954) mutant in L4 sleep stage.

Project description:To investigate which genes are up- or down-regulated during L4 sleep in C. elegans mutant aptf-1(gk794) we performed whole genome microarray expression profiling using the C. elegans (V2) Gene Expression Microarray, 4x44K from Agilent Technologies. Approximately 200 sleeping L4 worms were used per sample. As a control we have used N2 C. elegans wild type strain. Mutant condition (aptf-1(gk794)) and control (N2) were done in four replicates.

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Project description:To investigate which genes are up- or down-regulated by aptf-1 transcription factor in C. elegans, aptf-1(gk794) mutants pretzel-stage embryos were used to performe whole genome microarray expression profiling using the C. elegans (V2) Gene Expression Microarray, 4x44K from Agilent Technologies. Approximately 3000 pretzel-stage embryos were used per sample. As a control we have used N2 C. elegans wild type strain. Mutant condition (aptf-1(gk794)) and control (N2) were done in four replicates.

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Project description:Transcriptional profiling of C. elegans aptf-1(gk7954) mutant in L4 sleep stage.

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