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Gene Expression of Double Positive LCK:Hdac3 KO 3w old mice

ABSTRACT: Analysis of gene expression in double positive (DP) thymoctes from 3w old LCK Hdac3 conditional KO mice. In this data set we sorted GFP double positive (DP) thymocytes from 3w old mice to identify possible signaling and transcripitonal programs that might explain the DP to single positive block in cell maturation

ORGANISM(S): Mus musculus

PROVIDER: GSE73533 | GEO | 2015/09/29

SECONDARY ACCESSION(S): PRJNA297234

REPOSITORIES: GEO

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Histone Deacetylase 3 is required for efficient T cell development

Project description:Hdac3 is an important target of HDAC inhibitors used in the treatment of cutaneous T cell lymphoma. In order to gain an understanding of Hdac3 function in T cells,we deleted Hdac3 from early mouse thymocytes using LCK-Cre. Hdac3 deletion resulted in a loss of single positive thymocytes due to a defect in positive selection at the double positive (DP) stage of thymocyte development. To better characterize this defect, we sorted the DP1 and DP2 populations to for gene expression profiling. Total RNA was extracted from DP1 (GFP+CD4+CD8+CD5loTCRblo) or DP2 (GFP+CD4+CD8+CD5hiTCRbint) thymocytes isolated by FACS from Hdac3+/+ or Hdac3F/F LCK-Cre+ animals. Libraries were constructed from rRNA-depleted total RNA pools to identify altered gene expression in DP populations following Hdac3 deletion.

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Histone Deacetylase 3 is required for efficient T cell development

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HDAC3 restrains cytokine signaling during thymocyte development for the generation of CD4 T cells (ChIP-Seq)

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LCK-mediated RIPK3 Activation Controls Double-Positive Thymocyte Proliferation and Restrains Thymic Lymphoma by Regulating the PP2A-ERK Axis

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Project description:Gene Expression of Double Positive LCK:Hdac3 KO 3w old mice

| PRJNA297234 | ENA

Expression data for Lck-Cre, Med23flox/flox and Med23flox/flox;Lck-Cre thymocytes +/- 3hr exposure to plate bound anti-CD3 antibody

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2014-07-30 | E-GEOD-57061 | biostudies-arrayexpress

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