Project description:Atf1 was overexpressed in wt S. pombe cells for 24 hours and gene expression changes were analysed all samples were processed in duplicates. Gene expression in cells overexpressing ATf1 was compared with respect to the levels in control cells transformed with the empty vector.
Project description:RNA sequencing of total RNA purified from wild type S. pombe cells after 0, 5 and 24 hours culture in YES containing 100% D2O (deuterium oxide).
Project description:This RNA-Seq analysis compares gene expression of the pho7∆ fission yeast (Schizosaccharomyces pombe) strain prior to phosphate starvation (0 HR), i.e. in phosphate replete conditions, and at various times after phosphate starvation (4, 8, 12, 24, 36, and 48 HR) contrasting to the WT fission yeast S. pombe cells grown in phosphate replete conditions (WT 0HR)
Project description:KLF6 was overexpressed using an adenoviral delivery system. Overexpression was confirmed at 12, 24, 48 and 72 hours post infection by RT-PCR and Western Blot analysis. Codelink Whole genome microarrays were used to analyse changes in expression resulting from KLF6 overexpression.
Project description:Polyploidy has been implicated in genome instability and tumorigenesis. We use Schizosaccharomyces pombe diploids as a model for studying the consequences of whole genome duplications on genome integrity. In this study, our aim is to investigate the transcriptional profile between haploid and diploid S. pombe in unperturbed and MMS treated conditions (0.0075% MMS, 4 hours @ 32 degrees Celsius in YES media).
Project description:We performed lariat sequencing to profile the diversity of spliced RNA lariats in S. pombe identify annotated and alternate introns. Three different growth conditions were used to grow S. pombe wt and S. pombe Δdbr1. Lariat sequencing of the Δdbr1 strains and RNAseq of the wt and Δdbr1 strains were done to profile intron lariats and exon-exon junctions in RNA transcripts.
Project description:Human Notch1 intracellular domain (NICD) was overexpressed in human primary lymphatic endothelial cells (LECs) for 10 and 24 hours by adenovirus. A GFP-control adenovirus-infected cells (24hours) and uninfected cells were also analysed as controls. Total RNAs were harvested and subjected to Affymetrix U133A microarray.