Dataset Information

The developmental transcriptome of Dictyocaulus viviparus

ABSTRACT: Purpose: The goal of this study was to track fluctuations in gene expression across all defined life cycle stages of the cattle lungworm Dictyocaulus viviparus. Methods: Batches of worms from a Hannover, Germany strain were harvested in duplicate as eggs, L1, L2, L3, L4, hypobiotic L5, mixed-sex L5, male L5, female L5, mature males and mature females. RNA was isolated, reverse transcribed and sequenced on the Illumina platform. Finally, reads were mapped to the D. viviparus draft genome assembly and read counts associated with each feature were used to estimate gene expression levels and predict differential gene expression at various transitional points across the life cycle. Results: Principal component analyses indicated that major gene expression shifts occur upon hatching, infection of the bovid host, and sexual maturation.

ORGANISM(S): Dictyocaulus viviparus

PROVIDER: GSE73863 | GEO | 2015/10/09

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Down syndrome (DS) is the most prevalent genetic abnormality, occurring in ~1/700 live births caused by triplication of human chromosome 21 (HSA21). DS individuals have a multitude of phenotypic changes in peripheral systems and most notably, are cognitively impaired, with deficits in learning, language and memory acquisition and consolidation. Further, individuals with DS develop Alzheimer’s disease (AD) pathology during early middle-age (mid-30’s), although the underlying mechanism(s) driving this pathology onset are not well understood. Contributing to cognitive impairment in DS, morphological deficits are present in cortical lamination and distribution, with Layer III (L3) and Layer V (L5) pyramidal neurons showing reduced proliferation and a disorganized laminar structure. As such, examining cortical L3 and L5 pyramidal neurons in DS individuals with AD pathology may elucidate alternate driver mechanisms of disease onset. We examined DS and age-matched control (CTR) brains from individuals without intellectual disabilities or dementia using postmortem frontal cortex (BA9) via single population low input RNA-sequencing. We specifically targeted L3 and L5 pyramidal neurons to understand circuitry-based alterations in DS/AD individuals to elucidate mechanistic drivers of disease pathology. We show convergent and divergent gene expression changes in L3 and L5 pyramidal neurons which may underlie cognitive deficits and degenerative pathology associated with aging in the DS brain. We pinpoint alterations in HSA21 gene expression as well as a multitude of convergent differentially expressed genes (DEGs) and relevant pathways via bioinformatic inquiry in the DS brains that underlie mechanisms of degeneration. Distinctly, convergent gene expression reveals a neurotoxic phenotype, with multiple canonical pathways of dysregulation and disease functions linked to the convergent DEGs, including downregulation of the CLEAR signaling pathway and significantly increased activation of the neuroinflammation signaling pathway. We identify several target genes including mitogen activated protein kinase 1 and 3 (MAPK1/3), calcium voltage-gated channel subunit alpha1 A (CACNA1A) and superoxide dismutase 1 (SOD1), which exhibit overlap in multiple pathways and disease functions and/or display dysfunctional protein-protein network interactions for further examination. Novel targets identified by our single population approach in L3 and L5 pyramidal neurons may be drivers of the disease mechanism and therefore may be therapeutic candidates for amelioration of degenerative pathology that targets memory and executive function circuits in DS/AD.

Dataset Information

The developmental transcriptome of Dictyocaulus viviparus

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets