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Transcriptome comparison between Bacillus cereus ATCC 10987 cells with and without iron starvation

ABSTRACT: In this study we compared the transcriptomic profiles of cells grown in BHI, BHI supplemented with FeCl3, BHI supplemented with iron scavenger Bipyridine and both iron and the scavenger.

ORGANISM(S): Bacillus cereus ATCC 10987

PROVIDER: GSE74045 | GEO | 2016/05/31

SECONDARY ACCESSION(S): PRJNA298862

REPOSITORIES: GEO

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Transcriptome comparison between Bacillus cereus ATCC 10987 cells with and without iron starvation

Project description:In this study we compared the transcriptomic profiles of cells grown in BHI, BHI supplemented with FeCl3, BHI supplemented with iron scavenger Bipyridine and both iron and the scavenger. Liquid cultures grown in above mentioned four conditions with different iron availability were sampled at 5 h (Exponential growth) and 12 h (stationary phase start) of growth

2016-05-31 | E-GEOD-74045 | biostudies-arrayexpress

Global gene expression in Moraxella catarrhalis ATCC 43617 grown under iron-replete and iron-limiting conditions

Project description:Total RNA samples were isolated from M. catarrhalis grown in BHI (iron-replete condition, designated DF0) and in BHI containing 30 mM Desferal (iron-limiting condition, designated DF30). Total RNA and M. catarrhalis genome-directed primers (GDPs) were used for synthesis of Cy3- or Cy5-labeled cDNA samples. Three independent M. catarrhalis growth experiments and total RNA isolations were used for synthesis of cDNA samples, and a total of six DNA microarray hybridizations were performed in this study. Keywords: Effect of Growth Environment on Global Gene Expression by Moraxella catarrhalis

2007-12-07 | GSE4346 | GEO

Transcriptional Response to Iron Limitation in Pelagibacter ubique

Project description:We investigated the gene expression responses of Candidatus Pelagibacter ubique cultures to iron limitation. Differential expression was observed for genes in iron acquisition and incorporation operons. SfuC in particular was 16 times higher in iron-limited cultures and encodes a periplasmic iron-binding protein. Six natural seawater cultures were amended with minimal nutrients and inoculated with P. ubique. Close to maximum cell density, all carboys were supplemented with 100 nM ferrichrome (an iron-chelating siderophore) and three carboys were additionally supplemented with 1 µM FeCl3. Each of the six carboys was sampled for microarray analyses one, two, and eleven days after the ferrichrome addition.

2010-05-05 | E-GEOD-20962 | biostudies-arrayexpress

Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(II) chloride

Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing iron(II) chloride supplemented grown culture against non-iron treated grown culture in M9 minimal media Two-condition experiment, iron(II) chloride supplemented culture versus non-iron treated culture. 4 biological replicates including 3 technical replicates for one of the biological replicates. Swap-dye experiments were performed

2011-05-16 | E-GEOD-29319 | biostudies-arrayexpress

Transcription profiling of Bacillus anthracis Sterne during iron starvation

Project description:Effects of iron starvation on the transcriptional profile of Bacillus anthracis

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Characterization of germination receptors of Bacillus cereus ATCC 14579.

Project description:Specific amino acids, purine ribonucleosides, or a combination of the two is required for efficient germination of endospores of Bacillus cereus ATCC 14579. A survey including 20 different amino acids showed that l-alanine, l-cysteine, l-threonine, and l-glutamine are capable of initiating the germination of endospores of B. cereus ATCC 14579. In addition, the purine ribonucleosides inosine and adenosine can trigger germination of the spores. Advanced annotation of the B. cereus ATCC 14579 genome revealed the presence of seven putative germination (ger) operons, termed gerG, gerI, gerK, gerL, gerQ, gerR, and gerS. To determine the role of the encoded putative receptors in nutrient-induced germination, disruption mutants were constructed by the insertion of pMUTIN4 into each of the seven operons. Four of the seven mutants were affected in the germination response to amino acids or purine ribonucleosides, whereas no phenotype could be attributed to the mutants with disrupted gerK, gerL, and gerS loci. The strain with a disrupted gerR operon was severely hampered in the ability to germinate: germination occurred in response to l-glutamine but not in the presence of any of the other amino acids tested. The gerG mutant showed significantly reduced l-glutamine-induced germination, which points to a role of this receptor in the l-glutamine germination signaling pathway. gerR, gerI, and gerQ mutants showed reduced germination rates in the presence of inosine, suggesting a role for these operons in ribonucleoside signaling. Efficient germination by the combination of l-glutamine and inosine was shown to involve the gerG and gerI operons, since the germination of mutants lacking either one of these receptors was significantly reduced. Germination triggered by the combination of l-phenylalanine and inosine was lost in the gerI mutant, indicating that both molecules are effective at the GerI receptor.

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Transcriptome analysis of Bacillus cereus strain INRA C3 in response to iron depletion and lactoferrin, accomplished by RNA sequencing

Project description:The iron-binding glycoprotein lactoferrin exhibits anti-bacterial activity. To investigate the overall response of Bacillus cereus to lactoferrin, total transcriptome analyses were performed. Growth conditions were mMOD minimal medium, minimal medium plus 10 mg/ml lactoferrin, as well as minimal medium without iron source for comparison. Differential regulation of genes involved in iron acquisition as well as genes encoding enterotoxins and further virulence factors was of special interest.

2023-10-01 | GSE241250 | GEO

The Biosynthesis of UDP-D-QuiNAc in Bacillus cereus ATCC 14579.

Project description:N-acetylquinovosamine (2-acetamido-2,6-di-deoxy-D-glucose, QuiNAc) is a relatively rare amino sugar residue found in glycans of few pathogenic gram-negative bacteria where it can play a role in infection. However, little is known about QuiNAc-related polysaccharides in gram-positive bacteria. In a routine screen for bacillus glycan grown at defined medium, it was surprising to identify a QuiNAc residue in polysaccharides isolated from this gram-positive bacterium. To gain insight into the biosynthesis of these glycans, we report the identification of an operon in Bacillus cereus ATCC 14579 that contains two genes encoding activities not previously described in gram-positive bacteria. One gene encodes a UDP-N-acetylglucosamine C4,6-dehydratase, (abbreviated Pdeg) that converts UDP-GlcNAc to UDP-4-keto-4,6-D-deoxy-GlcNAc (UDP-2-acetamido-2,6-dideoxy-α-D-xylo-4-hexulose); and the second encodes a UDP-4-reductase (abbr. Preq) that converts UDP-4-keto-4,6-D-deoxy-GlcNAc to UDP-N-acetyl-quinovosamine in the presence of NADPH. Biochemical studies established that the sequential Pdeg and Preq reaction product is UDP-D-QuiNAc as determined by mass spectrometry and one- and two-dimensional NMR experiments. Also, unambiguous evidence for the conversions of the dehydratase product, UDP-α-D-4-keto-4,6-deoxy-GlcNAc, to UDP-α-D-QuiNAc was obtained using real-time 1H-NMR spectroscopy and mass spectrometry. The two genes overlap by 4 nucleotides and similar operon organization and identical gene sequences were also identified in a few other Bacillus species suggesting they may have similar roles in the lifecycle of this class of bacteria important to human health. Our results provide new information about the ability of Bacilli to form UDP-QuiNAc and will provide insight to evaluate their role in the biology of Bacillus.

| S-EPMC4514872 | biostudies-literature

Bacillus cereus ATCC 10987

Project description:Transcriptome comparison between Bacillus cereus ATCC 10987 cells with and without iron starvation

| PRJNA298862 | ENA

The effect of Iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5: iron(III) chloride

Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing iron(III) chloride supplemented grown culture against non-iron treated grown culture in M9 minimal media

2011-05-16 | GSE29320 | GEO

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