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Late stages of T-cell maturation in the thymus involve NF-κB and tonic type I interferon signaling

ABSTRACT: Positive selection occurs in the thymic cortex, but critical maturation events occur later in the medulla. We defined the precise stage at which T cells acquire competence to proliferate and emigrate. Transcriptome analysis of late gene changes suggested roles for NF-κB and interferon signaling. Mice lacking the IKK kinase TAK1, showed normal positive selection, but a specific block in functional maturation. NF-κB signaling provided protection from TNF, and was required for proliferation and emigration. Alternatively, the interferon signature was independent of NF-κB, and IFNαR deficient thymocytes showed reduced STAT1 levels and phenotypic abnormality, but were competent to proliferate. Thus, both NF-κB and tonic IFN signals are involved in the final maturation of thymocytes into naïve T cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE74078 | GEO | 2016/04/03

SECONDARY ACCESSION(S): PRJNA298913

REPOSITORIES: GEO

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