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Cryptococcus neoformans gene expression in minimal media of wild type (WT) and ALL2 mutant (all2Δ) cells

ABSTRACT: To examine the comparative transcription profile of WT and all2Δ mutant cells in in vitro conditions to determine genes responsible for hypervirulent phenotype of all2Δ cells. Using transcriptional profiling, we determined that many genes involved in transport were differentially transcribed between the wild type and the mutant strain. With the help of further experiments we could rule out that ALL2 has a unique function in maintaining the intracellular pH under acidic conditions.

ORGANISM(S): Cryptococcus neoformans

PROVIDER: GSE74217 | GEO | 2015/12/09

SECONDARY ACCESSION(S): PRJNA299668

REPOSITORIES: GEO

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Cryptococcus neoformans gene expression in minimal media of wild type (WT) and ALL2 mutant (all2Î) cells

Project description:To examine the comparative transcription profile of WT and all2Î mutant cells in in vitro conditions to determine genes responsible for hypervirulent phenotype of all2Î cells. Using transcriptional profiling, we determined that many genes involved in transport were differentially transcribed between the wild type and the mutant strain. With the help of further experiments we could rule out that ALL2 has a unique function in maintaining the intracellular pH under acidic conditions. For arrays, total RNA was isolated from WT and all2Î mutant using Qiagen RNeasy Kit. For RNA extraction, WT and all2Î mutant were grown in minimal media. Cells were grown for overnight, diluted 1:100 and then again grown overnight or up to an OD of 0.6-0.7 with agitation (150rpm) at 37Â°C.

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Project description:To examine the comparative transcription profile of WT and all1all2Î mutant cells in in vitro conditions to determine genes resposible for hyporvirulent phenotype of all1all2Î mutant cells. Using transcriptional profiling, we determined that many genes involved in amino acid transport were differentially transcribed between the wild type and the all1all2Î mutant strain. The genes differentially expressed in double mutant of ALL1 ALL2 did not overlap with ALL1 and ALL2 comparative transcriptomes with RC2 (WT). For arrays, total RNA was isolated from WT and all1all2Î mutant using Qiagen RNeasy Kit. For RNA extraction, WT and all1all2Î mutant were grown in minimal media. Cells were grown for overnight, diluted 1:100 and then again grown overnight or up to an OD of 0.6-0.7 with agitation (150rpm) at 37Â°C.

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