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Gene expression profiles in rat lung following intratracheal instillation with short size single-wall and multi-wall carbon nanotubes

ABSTRACT: To further development of our gene expression approach to assess the effects of manufactured nanomaterials at the transcriptional level, we have employed whole genome microarray expression profiling to discuss the differences of the pulmonary and pleural

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE75148 | GEO | 2015/11/19

SECONDARY ACCESSION(S): PRJNA302639

REPOSITORIES: GEO

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Gene expression profiles in rat lung following intratracheal instillation with short size single-wall and multi-wall carbon nanotubes

Project description:To further development of our gene expression approach to assess the effects of manufactured nanomaterials at the transcriptional level, we have employed whole genome microarray expression profiling to discuss the differences of the pulmonary and pleural Groups of nine-week-old male Wistar rats (n = 4 per group/ time point) were intratracheally instilled with short SWCNTs and MWCNTs suspended in 0.4 mL of 10mg/mL bovine serum albumin (BSA) as a single injection, respectively. Control groups received 10mg/

2015-11-19 | E-GEOD-75148 | biostudies-arrayexpress

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Gene expression profiles in rat lung following intratracheal instillation with impurity-free single-wall carbon nanotubes

Project description:To further development of our gene expression approach to assess the effects of manufactured nanomaterials at the transcriptional level, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish characterization of physicochemical properties of impurity-free single-wall carbon nanotubes (SWCNTs). We have prepared two types of dispersed the SWCNTs, namely relatively small bundles and a short linear shape (CNT-1) and large bundles and a long linear shape (CNT-2), and attempted to characterize time-dependent changes in the gene expression of lung tissues until 90 days after intratracheal instillation with SWCNTs suspensions at 0.4 mg injected dose per rat. Groups of nine-week-old male Wistar rats (n= 4 per group/ time point) were intratracheally instilled with single-wall carbon nanotubes (SWCNTs) suspended in 0.4 ml of 1.0mg/mL bovine serum albumin (BSA) as a single injection 0.4 mg SWCNTs/ rat. Control groups received 1.0mg/mL BSA (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 M-BM-0C with a chow diet ad libitum, and dissected at 1day, 3 days, 7 days, 30 days, and 90 days post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.

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Gene expression profiles in the lung following intratracheal instillation of cellulose nanofibrils and multi-walled carbon nanotubes in a murine COPD model

Project description:To evaluate the transcriptional-level biological effects of manufactured nanomaterials, a whole-genome microarray analysis was performed on the lungs of a murine chronic obstructive pulmonary disease (COPD) model. Mice were intratracheally instilled with cellulose nanofibrils (CNFs) or multi-walled carbon nanotubes (MWCNTs).

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Gene expression profiles in rat lung following intratracheal instillation with single-wall carbon nanotubes

Project description:Gene expression profiling of the rat lung following intratracheal instillation with single-wall carbon nanotubes (SWCNTs) was employed to gain insights into these molecular events. We attempted to characterize time-dependent changes in the gene expression until 754 days after intratracheal instillation with SWCNTs suspensions at 0.2 mg (L-SWCNT) and 0.4 mg (H-SWCNT) injected dose per rat, and to identify the shift from the acute-phase to the chronic-phase phase on the basis of evaluation at the molecular level.

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Gene expression profiles in rat alveolar macrophages exposure to multi-walled carbon nanotubes

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Intratracheal instillation of single-wall carbon nanotubes in the rat lung induces time-dependent changes in gene expression.

Project description:The use of carbon nanotubes in the industry has grown; however, little is known about their toxicological mechanism of action. Single-wall carbon nanotube (SWCNT) suspensions were administered by single intratracheal instillation in rats. Persistence of alveolar macrophage-containing granuloma was observed around the sites of SWCNT aggregation at 90 days post-instillation in 0.2-mg- or 0.4-mg-injected doses per rat. Meanwhile, gene expression profiling revealed that a large number of genes involved in the inflammatory response were markedly upregulated until 90 days or 180 days post-instillation. Subsequently, gene expression patterns were dramatically altered at 365 days post-instillation, and the number of upregulated genes involved in the inflammatory response was reduced. These results suggested that alveolar macrophage-containing granuloma reflected a characteristic of the histopathological transition period from the acute-phase to the subchronic-phase of inflammation, as well as pulmonary acute phase response persistence up to 90 or 180 days after intratracheal instillation in this experimental setting. The expression levels of the genes Ctsk, Gcgr, Gpnmb, Lilrb4, Marco, Mreg, Mt3, Padi1, Slc26a4, Spp1, Tnfsf4 and Trem2 were persistently upregulated in a dose-dependent manner until 365 days post-instillation. In addition, the expression levels of Atp6v0d2, Lpo, Mmp7, Mmp12 and Rnase9 were significantly upregulated until 754 days post-instillation. We propose that these persistently upregulated genes in the chronic-phase response following the acute-phase response act as potential biomarkers in lung tissue after SWCNT instillation. This study provides further insight into the time-dependent changes in genomic expression associated with the pulmonary toxicity of SWCNTs.

| S-EPMC4487535 | biostudies-literature

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