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Identification of the long, edited dsRNAome in LPS-stimulated primary human peripheral blood monocytes

ABSTRACT: Endogenous double-stranded RNA (dsRNA) is intricately regulated in mammals to prevent aberrant activation of host inflammatory pathways by cytosolic dsRNA binding proteins. We define the endogenous dsRNA repertoire in human peripheral blood monocytes using published data (GSE60216) derived from 18 RNA-Seq experiments from neonates, adults, and elderly patients during the inflammatory response to bacterial lipopolysaccharide. Editing by adenosine deaminases that act of RNA (ADAR) enzymes was quantified temporally using this RNA-Seq data to identify 3,438 Editing Enriched Regions (EERs), indicative of highly structured dsRNA. Nearly all human EERs were present in introns. A comparison of EERs from mouse and human revealed significant conservation of EERs between mouse EER- associated genes and homologous human genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE75149 | GEO | 2016/04/14

SECONDARY ACCESSION(S): PRJNA302642

REPOSITORIES: GEO

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Identification of the long, edited dsRNAome in LPS-stimulated bone marrow-derived macrophages (BMDMs)

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