Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).

ABSTRACT: To identify the miRNAs which regulate the decidualization of ECSCs, we explored differentially expressed miRNAs between untreated ECSCs (n=4) and decidualized ECSCs (n=4) using miRNA microarray. The miRNA expression profiles in untreated ECSCs and decidualized ECSCs were obtained, then the miRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 17% of cases have good flags). We established the criteria for regulated genes: ratio ≥2.0-fold for up-regulated genes and ratio ≤0.5 for down-regulated genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE75422 | GEO | 2016/10/12

SECONDARY ACCESSION(S): PRJNA304174

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

mRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).

Project description:To identify the mRNAs which regulate the decidualization of ECSCs, we explored differentially expressed mRNAs between untreated ECSCs (n=4) and decidualized ECSCs (n=4) using gene expression microarray. The mRNA expression profiles in untreated ECSCs and decidualized ECSCs were obtained, then the mRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 58% of cases have good flags). We established the criteria for regulated genes: Z-score ≥2.0 and ratio ≥2.0-fold for up-regulated genes, and Z-score ≤–2.0 and ratio ≤0.5 for down-regulated genes.

2016-10-12 | GSE75423 | GEO

miRNA expression profiles in decidualized and non-decidualized normal endometrial stromal cells (NESCs).

Project description:To identify the miRNAs which regulate endometrial decidualization, we explored differentially expressed miRNAs between untreated NESCs (n=4) and decidualized NESCs (n=4) using miRNA microarray. The miRNA expression profiles of untreated NESCs and decidualized NESCs were obtained, then the miRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 13% of cases have good flags). We established the criteria for regulated genes: ratio ≥2.0-fold for up-regulated genes and ratio ≤0.5 for down-regulated genes.

2016-10-12 | GSE75424 | GEO

mRNA expression profiles in decidualized and non-decidualized normal endometrial stromal cells (NESCs).

Project description:To identify the mRNAs which regulate endomrtial decidualization, we explored differentially expressed mRNAs between untreated NESCs (n=4) and decidualized NESCs (n=4) using gene expression microarray. The mRNA expression profiles of untreated in NESCs and decidualized NESCs were obtained, then the mRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 43% of cases have good flags). We established the criteria for regulated genes: Z-score ≥2.0 and ratio ≥2.0-fold for up-regulated genes, and Z-score ≤–2.0 and ratio ≤0.5 for down-regulated genes.

2016-10-12 | GSE75425 | GEO

miRNA expression profiles in endometriotic cyst stromal cells (ECSCs).

2013-02-06 | GSE40186 | GEO

miRNA expression profiles in endometriotic cyst stromal cells (ECSCs).

Project description:To identify the miRNAs which play a role in the development of endometriosis, we explored differentially expressed miRNAs between ECSCs (N=8) and normal endometrial stromal cells (NESCs, n=8) using miRNA microarray. The expression profiles in ECSCs and NESCs were obtained, then the miRNAs were filtered by signal intensity (20.0<, at least one out of 16 samples have values within range) and flags (at least 70% of cases have good flags). The differentially expressed miRNAs in ECSCs compared with NESCs were identified using volcano plot (Fold change >= 2.0, Mann-Whiteny U test p<0.05). The primary culture of ECSCs and NESCs (controls) were utilized. Total RNA was extracted from them, and miRNA microarray analysis was performed. The miRNAs differentially expressed between ECSCs and NESCs were identified, and aberrantly expressed miRNAs in ECSCs were defined as the endometriosis related miRNAs.

2013-02-06 | E-GEOD-40186 | biostudies-arrayexpress

Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs)

Project description:Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs)

| PRJNA304176 | ENA

Gene expression profiles of decidualized human endometrial stromal cells knockdown for HOXA10

Project description:The homeobox gene HOXA10 plays a key role in endometrial differentiation during embryogenesis and is abundantly expressed in the adult endometrium and decidual cells. In the adult endometrium the expression of HOXA10 is regulated by estrogen and progesterone and the levels are highest in the decidualized cells. We have shown that HOXA10 is required in the endometrial cells to maintain the decidual cell phenotype. In this study we aimed to determine the molecular mechanisms by which HOXA10 maintains the decidual phenotype and the other roles it might have in decidual physiology. In vitro decidualized human endometrial stromal cells were knocked down for HOXA10 using siRNA and gene expression profiles of the scrambled and HOXA10 siRNA transfected cells were compared at 24, 48 and 72 h post transfection. Several genes having multiple functions were found to be differentially expressed. We postulate that HOXA10 is required by the decidual cells to support immune cell differentiation, trophoblast invasion and cellular remodeling. In vitro decidualized priamry cultures of human endometrial strmal cells transfected with a siRNA againts HOXA10 or a scrambled siRNA. Cells harvested at 24, 48 and 72 h post transfection. Dual channel hybridization with dye swap design in biological replicates

2012-02-14 | E-GEOD-35801 | biostudies-arrayexpress

Genome-wide gene expression analysis of new cardiomyocytes (CMs) derived from resident c-kitpos endogenous cardiac stem cells (eCSCs) after myocardial injury by Isoproterenol (ISO) in mice.

2013-07-30 | GSE49318 | GEO

Genome-wide gene expression analysis of new cardiomyocytes (CMs) derived from resident c-kitpos endogenous cardiac stem cells (eCSCs) after myocardial injury by Isoproterenol (ISO) in mice.

Project description:Genome-wide gene expression analysis of new cardiomyocytes (CMs) derived from resident c-kitpos endogenous cardiac stem cells (eCSCs) after myocardial injury by Isoproterenol (ISO) in mice. These data show that of new CMs derived from resident c-kitpos eCSCs have a gene expression profile that closely resembles the specific gene expression of adult cardiomyocytes. This result supports a role of c-kitpos eCSCs in the regeneration and repair of myocardial damage. To test the identity and the degree of differentiation of new cardiomyocytes (CMs) derived from resident c-kitpos endogenous cardiac stem cells (eCSCs) after myocardial injury by Isoproterenol (ISO) in mice. Global gene expression profiles by microarray was obtained in c-kitpos eCSCs, c-kitpos eCSC-derived YFPpos CMs and normal adult CMs. c-kitposCD45neg eCSCs were isolated from B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J mice and harvested at 4th passage (n=3). YFPpos cardiomyocytes were FACS sorted from left ventricle apex of Lentirius-c-kit/cre recombined B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J mice 28 days after ISO (N=3). Normal adult CMs were isolated from B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J mice (n=3).

2013-07-30 | E-GEOD-49318 | biostudies-arrayexpress

Homo sapiens

Project description:miRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).

| PRJNA304174 | ENA