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Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs)

ABSTRACT: Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs)

PROVIDER: PRJNA304176 | ENA |

REPOSITORIES: ENA

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miRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).

Project description:To identify the miRNAs which regulate the decidualization of ECSCs, we explored differentially expressed miRNAs between untreated ECSCs (n=4) and decidualized ECSCs (n=4) using miRNA microarray. The miRNA expression profiles in untreated ECSCs and decidualized ECSCs were obtained, then the miRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 17% of cases have good flags). We established the criteria for regulated genes: ratio ≥2.0-fold for up-regulated genes and ratio ≤0.5 for down-regulated genes.

2016-10-12 | GSE75422 | GEO

mRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).

Project description:To identify the mRNAs which regulate the decidualization of ECSCs, we explored differentially expressed mRNAs between untreated ECSCs (n=4) and decidualized ECSCs (n=4) using gene expression microarray. The mRNA expression profiles in untreated ECSCs and decidualized ECSCs were obtained, then the mRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 58% of cases have good flags). We established the criteria for regulated genes: Z-score ≥2.0 and ratio ≥2.0-fold for up-regulated genes, and Z-score ≤–2.0 and ratio ≤0.5 for down-regulated genes.

2016-10-12 | GSE75423 | GEO

Observation of the Changes in Chemokine-related Gene Expression of Endometriotic Cyst Stromal Cells by Three-dimensional Collagen Gel Culture

Project description:Endometriotic cyst stromal cells (ECSCs) were isolated from ovarian endometriotic cyst and cultured for 48 hours in the floating three-dimensional collagen gel culture or in the conventional two-dimensional culture. Total mRNAs were extracted and subjected to gene expression microarray.

2020-07-31 | GSE136412 | GEO

miRNA expression profiles in endometriotic cyst stromal cells (ECSCs).

Project description:To identify the miRNAs which play a role in the development of endometriosis, we explored differentially expressed miRNAs between ECSCs (N=8) and normal endometrial stromal cells (NESCs, n=8) using miRNA microarray. The expression profiles in ECSCs and NESCs were obtained, then the miRNAs were filtered by signal intensity (20.0<, at least one out of 16 samples have values within range) and flags (at least 70% of cases have good flags). The differentially expressed miRNAs in ECSCs compared with NESCs were identified using volcano plot (Fold change >= 2.0, Mann-Whiteny U test p<0.05). The primary culture of ECSCs and NESCs (controls) were utilized. Total RNA was extracted from them, and miRNA microarray analysis was performed. The miRNAs differentially expressed between ECSCs and NESCs were identified, and aberrantly expressed miRNAs in ECSCs were defined as the endometriosis related miRNAs.

2013-02-06 | E-GEOD-40186 | biostudies-arrayexpress

miRNA expression profiles in endometriotic cyst stromal cells (ECSCs).

2013-02-06 | GSE40186 | GEO

Homo sapiens

Project description:mRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).

| PRJNA304173 | ENA

Homo sapiens

Project description:miRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).

| PRJNA304174 | ENA

Transcriptome analysis of endometriotic stromal cells treated with hydrogen peroxide

Project description:In ovarian endometrioma, much iron, which is derived from blood in cyst, deposit in stroma. This iron generates oxidative stress by Fenton reaction, which is supposed to affect endometriotic stromal cells. We used gene expression microarrays to find influence of oxidative stress on endometriotic stromal cells. Gene expression microarrays revealed no statistically differences caused by oxidative stress.

2012-06-04 | GSE31515 | GEO

CCAAT/enhancer-binding protein alpha is epigenetically silenced by histone acetylation in endometriosis and promotes the pathogenesis of endometriosis: a novel therapeutic target

Project description:Accumulating evidences suggest that various epigenetic aberrations play definite roles in the pathogenesis of endometriosis. The aim of this study is to identify 1) the panel of aberrantly expressed genes that are epigenetically suppressed by histone acetylation in endometriosis; 2) the roles of CCAAT/enhancer-binding protein (C/EBP) alpha, one of the candidate molecules whose expression was epigenetically repressed in endometriotic cyst stromal cells (ECSCs), in the pathogenesis of endometriosis; and 3) the efficacy of the histone deacetylase inhibitors for the treatment of endometriosis. Subconfluent ECSCs cultured in 10-cm dish were further incubated for 72 h with or without VPA (8 mM) and/or 5aza. Total RNA from untreated ECSCs (n=4), VPA-treated ECSCs (n=4), 5aza-treated ECSCs (n=4), and VPA- and 5aza-treated ECSCs (n=4) was extracted and subjected to gene expression microarray analysis.

2013-10-21 | E-GEOD-44207 | biostudies-arrayexpress

Global m6A-modified mRNAs in undecidualized and decidualized primary human endometrial stromal cells [meRIP-seq]

Project description:Global m6A-modified mRNAs in undecidualized and decidualized primary human endometrial stromal cells were analyzed by using methylated RNA immunoprecipitation sequencing (MeRIP-seq)

2024-04-14 | GSE263331 | GEO

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