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Effect of bovine viral diarrhoea virus infection in Madin-Darby bovine kidney cells

ABSTRACT: Analysis of gene expression induced in Madin-Darby bovine kidney cells (MDBK) infected with Bovine viral diarrhoea virus BVDV2 strain 1373. Keywords: other

ORGANISM(S): Bos taurus

PROVIDER: GSE756 | GEO | 2004/05/31

SECONDARY ACCESSION(S): PRJNA87705

REPOSITORIES: GEO

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Effect of bovine viral diarrhoea virus infection in Madin-Darby bovine kidney cells

Project description:Analysis of gene expression induced in Madin-Darby bovine kidney cells (MDBK) infected with Bovine viral diarrhoea virus BVDV2 strain 1373. Keywords: other

2004-05-31 | E-GEOD-756 | biostudies-arrayexpress

Transcriptome and Proteomic Analysis Reveals Up-Regulation of Innate Immunity Related Genes Expression in Caprine Herpesvirus 1 Infected Madin Darby Bovine Kidney Cells

Project description:In order to further understanding the replication and pathogenesis of Caprine herpesvirus 1 (CpHV-1) and virus-host interactions. In this study, the proteomics of CpHV-1 infected Madin Darby bovine kidney (MDBK) cells was explored by using iTRAQ-LC-MS/MS technology.

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Transcriptomic characterization of cattle primary hepatocytes and its comparison with Madin-Darby bovine kidney (MDBK) cells and liver tissue

Project description:Cattle primary cultured hepatocytes (CHs) have been used as a useful in vitro model for drug metabolism studies. However, they suffer some limitations, when compared with the ex vivo liver tissue, such as difficult adhesion to the substrate following isolation, a limited viability and a rapid loss of differentiated characteristics. Therefore, a reliable hepatic in vitro model is actually needed for cattle. As established bovine hepatic cell lines are not available, we characterized the cattle primary CHs' transcriptome by using a cDNA microarray approach and compared it with ex vivo cattle liver tissue as well as with the Madin-Darby Bovine Kidney (MDBK) cells (as an established cell line for transfection purposes).<br>This part of the study compares the primary CHs vs. liver tissue. The comparison of MDBK cells vs. the primary CHs is found under E-MTAB-4430.

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Differentially-expressed miRNAs in MDBK cells infected with bovine viral diarrhea virus compared to MDBK cells

Project description:Non-canonical microRNAs (miRNAs) are a class of short endogenous RNA molecules with the ability to control development, autophagy, apoptosis and the stress response in eukaryotes by pairing with partially complementary sites in the 3' untranslated regions (UTRs) of targeted genes. Recent studies have demonstrated that miRNAs serve as critical effectors in intricate networks of host-pathogen interactions. Thereforce, the differential expression of miRNAs were evaluated in Madin-Darby bovine kidney (MDBK) cells infected with bovine viral diarrhea virus (BVDV) NADL (100 TCID50/ 0.1 ml) for 6 h compared to normal MDBK cells using Solexa high-throughput sequencing technology (BGI, China). Examination of small RNA populations in BVDV infected MDBK cells compared to MDBK cells

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Differentially-expressed miRNAs in MDBK cells infected with bovine viral diarrhea virus compared to MDBK cells

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Expression data from HIF2A WT vs KD Madin-Darby canine kidney epithelial cells (MDCK strain II cells (ATCC:CCL-34)) under normoxic and hypoxic conditions

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Madin-Darby Canine Kidney (MDCK) cells grown in 2-dimensional and 3-dimensional type I collagen culture.

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