Translatome analysis of NB-LRR immune response identifies important contributors to plant immunity in Arabidopsis.
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ABSTRACT: An important branch of plant immunity involves the recognition of pathogens by the NB-LRR proteins encoded by disease resistance genes. However, signaling events downstream of NB-LRR activation are poorly understood. We have analyzed the Arabidopsis translatome using ribosome affinity purification and RNAseq. Our results show that the translational status of hundreds of genes are differentially affected upon activation of the NB-LRR protein RPM1, showing an overall pattern of a switch away from growth-related activities to defense. Among these is the central translational regulator and growth promoter, TOR kinase. Suppression of TOR expression leads to increased resistance to pathogens while overexpression of TOR results in increased susceptibility, indicating an important role for translational control in the growth to defense switch. Furthermore, we show that several additional genes whose mRNAs are translationally regulated, including BIG, CCT2 and CIPK5, are required for plant innate immunity, identifying novel actors in plant defense.
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE75640 | GEO | 2017/03/06
SECONDARY ACCESSION(S): PRJNA304946
REPOSITORIES: GEO
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