Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression analysis of Mycobacterium tuberculosis H37Rv biofilm after treatment with DTT ( Reduced) for 29 hrs

ABSTRACT: Expression profile of Mycobacterium tuberculosis H37Rv biofilm as induced by DTT (Reduced) 6mM

ORGANISM(S): Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv

PROVIDER: GSE75847 | GEO | 2016/02/08

SECONDARY ACCESSION(S): PRJNA305522

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression analysis of Mycobacterium tuberculosis H37Rv biofilm after treatment with DTT ( Reduced) for 29 hrs

Project description:Expression profile of Mycobacterium tuberculosis H37Rv biofilm as induced by DTT (Reduced) 6mM DTT reduced at 6 mM concentration was added to log phase culture of Mtb H37Rv. After 29 hours RNA was isolated and hybridization was done on microarrays

2016-02-08 | E-GEOD-75847 | biostudies-arrayexpress

Expression analysis of M.tb H37Rv after treatment with DTT (reduced) for 3 hrs

Project description:Effect of Thiol reductive stress (DTT 1mM) on M.tb Rv was studied. DTT reduced at 1 mM concentration was added to log pahse culture of M.tb H37Rv. After Three hours RNA was isolated and hybridization was done on HRI-UMNDJ Mycobacterium tuberculosis 4.8K CAG_Mtb microarrays.

2015-04-28 | E-GEOD-68350 | biostudies-arrayexpress

Expression analysis of M.tb H37Rv after treatment with DTT (reduced) for 3 hrs

Project description:Effect of Thiol reductive stress (DTT 1mM) on M.tb Rv was studied.

2015-04-28 | GSE68350 | GEO

Expression analysis of M. tb H37Rv after treatment with DTT for 3 hrs

Project description:Effect of Thiol reductive stress (DTT 5mM) on M.tb Rv was studied.

2016-04-14 | GSE77848 | GEO

Mycobacterium tuberculosis H37Rv

Project description:Expression analysis of Mycobacterium tuberculosis H37Rv biofilm after treatment with DTT ( Reduced) for 29 hrs

| PRJNA305522 | ENA

Mycobacterium tuberculosis H37Rv

Project description:Expression analysis of M. tb H37Rv after treatment with DTT for 3 hrs

| PRJNA311723 | ENA

Mycobacterium tuberculosis H37Rv

Project description:Expression analysis of M.tb H37Rv after treatment with DTT (reduced) for 3 hrs

| PRJNA282491 | ENA

Whole transcriptome profiling of H37Rv- and H37Rv△1759c-infected macrophages.

Project description:To explore the regulatory network of noncoding RNAs after M.tb infection and the role of Rv1759c in the infection process, we collected samples of H37Rv- and H37Rv△1759c-infected macrophages and explored the full transcriptome expression profile. We constructed DE-lncRNA/DE-circRNA-DE-miRNA-DE-mRNA regulatory networks during H37Rv and H37Rv△1759c infection. In addition, we first discovered the close relationship between Rv1759c and chemokines during M.tb infection by comparing the transcription profiles of H37Rv and H37Rv△1759c and bioinformatics analysis. Here, our study comprehensively characterizes the ncRNA and mRNA profiles in macrophages infected with H37Rv and H37Rv△1759c, which provides support and new directions for in-depth exploration of ncRNA and PE/PPE family functions during the infection process.

2021-09-30 | GSE184660 | GEO

M. tuberculosis H37Rv: mutant expressing foreign peptide SL3 Vs control H37Rv

Project description:Transcriptional profiling of M.tb H37Rv cells comparing control wild type H37Rv with H37Rv cells electroporated with constitutive expression plasmid pVV16 expressing ESAT-6 binding peptide SL3. The expression of SL3 makes H37Rv less virulent during ex vivo and BalB/c mice infections, sequesters ESAT-6 inside M.tb cells and cause severe defects in mycobacterial morphology. Goal was to determine the effects of SL3 expression on global H37Rv gene expression.

2014-04-10 | GSE51085 | GEO

Transcriptomic analysis of Wild type M.tb H37Rv, HigB KO M.tb H37Rv and complemented Hig B KO M.tb H37Rv

Project description:Gene expression microarray was performed using early log phase culture at 37 degree and 200 rpm followed by RNA extraction from M.tb H37Rv, HigB KO M.tb H37Rv and complemented Hig B KO M.tb H37Rv For gene expression profiling, total RNA was isolated from wild type H37Rv, higB KO mutant strain and complemented strains. The bacterial pellets for M.tb H37Rv wild type, M.tb H37Rv HigB KO mutant strain and complemented strain were resuspended in 1.0 ml TRIzol (Invitrogen Corporation, Carlsbad, CA); mRNA was extracted as per standard protocols and cleaned using RNAeasy columns (Qiagen GmbH, Hilden, Germany). Extracted mRNA (1 μg) was treated with DNase I using the Ambion DNA-free kit (Invitrogen Corporation, Carlsbad, CA) to remove genomic DNA contamination and quantified using Nanodrop 2000c spectrophotometer (Thermo Scientific, USA). The purity and integrity of RNA samples were assessed Agilent 2100 Bio analyzer (Agilent TechnologiesInc., USA). Further, 25ng of RNA was amplified and labeled using Low input Quick Amp WT Labeling kit (Agilent Technologies, USA). The labeled cRNA was purified using RNAeasy columns (Qiagen, USA) and total yields were quantified using Nanodrop 2000c spectrophotometer.

2021-11-30 | GSE179403 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data