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Expression data from fully-expanded leaflet of Bambara groundnut (S19-3 genotype)

ABSTRACT: Low temperature stress in a number of African countries, such as Botswana, at night can effect the growth and development of bambara groundnut, leading to losses in potential crop yield. Therefore, we made an attempt to identify and analyze the genes and gene modules associated with low temperature stress response in bambara groundnut using the cross-species microarray technique, as bambara groundnut has no microarray chip, coupled with network-based analysis.

ORGANISM(S): Vigna subterranea

PROVIDER: GSE75982 | GEO | 2016/02/10

SECONDARY ACCESSION(S): PRJNA305891

REPOSITORIES: GEO

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Expression data from fully-expanded leaflet of Bambara groundnut genotype (S19-3)

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Expression data from fully-expanded leaflet of Bambara groundnut genotype (S19-3)

Project description:Low temperature stress in a number of African countries, such as Botswana, at night can effect the growth and development of bambara groundnut, leading to losses in potential crop yield. Therefore, we made an attempt to identify and analyze the genes and gene modules associated with low temperature stress response in bambara groundnut using the cross-species microarray technique, as bambara groundnut has no microarray chip, coupled with network-based analysis. Three plants of the bambara groundnut genotype â??S19-3â?? were grown in controlled environment growth rooms at Sutton Bonington Campus, University of Nottingham under a 12 hour photoperiod and at a constant temperature of 27Â°C. Plants were grown in soil columns containing a growing medium of 1 part John Innes 2 compost to 1 part sand, and were watered as required. A single, fully-expanded leaflet was sampled from each of the three plants growing at 27Â°C, snap frozen in liquid nitrogen and stored at -80Â°C. Plants were given a further 3 days at 27Â°C to recover from sampling before being moved to a controlled environment room at 23Â°C. On the fifth day at 23Â°C a single fully-expanded leaflet was sampled and stored, as described above. After 3 further days plants were moved to 18Â°C, and then five days later were sampled again.

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Expression data from fully-expanded leaflet of Bambara groundnut (S19-3 genotype)

Project description:Low temperature stress in a number of African countries, such as Botswana, at night can effect the growth and development of bambara groundnut, leading to losses in potential crop yield. Therefore, we made an attempt to identify and analyze the genes and gene modules associated with low temperature stress response in bambara groundnut using the cross-species microarray technique, as bambara groundnut has no microarray chip, coupled with network-based analysis. Two plants of the bambara groundnut genotype âS19-3â were grown in controlled environment growth rooms at Sutton Bonington Campus, University of Nottingham under a 12 hour photoperiod and at a constant temperature of 27Â°C. Plants were grown in soil columns containing a growing medium of 1 part John Innes 2 compost to 1 part sand, and were watered as required. A single, fully-expanded leaflet was sampled from each of the three plants growing at 27Â°C, snap frozen in liquid nitrogen and stored at -80Â°C. Plants were given a further 3 days at 27Â°C to recover from sampling before being moved to a controlled environment room at 23Â°C. On the fifth day at 23Â°C a single fully-expanded leaflet was sampled and stored, as described above. After 3 further days plants were moved to 18Â°C, and then five days later were sampled again.

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