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Transcriptional profiling of Regulatory T-cells isolated from adult skin and skin draining lymph nodes (SDLNs)

ABSTRACT: Purpose: The goals of this study were to identify preferential gene expression signatures that are unique to telogen skin resident Tregs relative to peripheral Tregs Methods: Tregs from telogen skin and SDLNs were purified by cell sorting (using the Treg GFP reporter mouse line Foxp3-DTR/GFP) to generate mRNA transcription profiles. Results: Transcriptional profiling revealed a unique Skin treg signature relative to SDLN Tregs Conclusion: Our study represents the first detailed analysis of the skin Treg transcriptome.

ORGANISM(S): Mus musculus

PROVIDER: GSE76138 | GEO | 2017/04/28

SECONDARY ACCESSION(S): PRJNA306418

REPOSITORIES: GEO

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Transcriptional profiling of Regulatory T-cells isolated from adult skin and skin draining lymph nodes (SDLNs)

Project description:Purpose: The goals of this study were to identify preferential gene expression signatures that are unique to telogen skin resident Tregs relative to peripheral Tregs Methods: Tregs from telogen skin and SDLNs were purified by cell sorting (using the Treg GFP reporter mouse line Foxp3-DTR/GFP) to generate mRNA transcription profiles. Results: Transcriptional profiling revealed a unique Skin treg signature relative to SDLN Tregs Conclusion: Our study represents the first detailed analysis of the skin Treg transcriptome. mRNA profiles of skin and SDLN Tregs isolated from 6 week old Foxp3-DTR/GFP mice.

2017-04-28 | E-GEOD-76138 | biostudies-arrayexpress

Transcriptional profiling of Regulatory T-cells isolated from neonatal skin and skin draining lymph nodes (SDLNs)

Project description:Purpose: The goals of this study were to identify preferential gene expression signatures that are unique to Tregs in neonatal skin relative to peripheral Tregs Methods: Tregs from telogen skin and SDLNs were purified by cell sorting (using the Treg GFP reporter mouse line Foxp3-DTR/GFP) to generate mRNA transcription profiles. Results: Transcriptional profiling revealed a unique neonatal skin Treg signature relative to SDLN Tregs Conclusion: Our study represents the first detailed analysis of the neonatal skin Treg transcriptome.

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2021-10-27 | GSE180649 | GEO

Nonspecific induction of distinct regulatory T cell states in oncogene-driven hyperproliferative skin

Project description:Regulatory T cells (Tregs) recruited to peripheral tissues are associated with chronic diseases such as cancer. Tissue-specific signals and T cell receptor (TCR) specificity are critical to shape Treg phenotype diversity. We examined single-cell transcriptomic and TCR profiles of Tregs in human papillomavirus (HPV) E7-transformed hyperproliferative skin of mice. Tregs without evidence of E7 specificity were enriched in E7 transgenic skin grafts when compared with non-transgenic grafts. E7 transgenic skin grafts contained more Klrg1+ Tregs and Il1r2+ Tregs than non-transgenic grafts. These two Treg states had distinct effector phenotypes but shared a core gene signature with previously described tumour-infiltrating Tregs. Pseudotime trajectory of Tregs predicted phenotypic plasticity of TCR clonotypes, both within skin and between skin and draining lymph nodes. Together, we reveal that oncogene-driven hyperproliferative skin enables induction of nonspecific Tregs despite the presence of non-self antigen and Tregs exist in distinct cell states with unique effector gene signatures.

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Regulatory T Cell-Microglia Crosstalk Promotes White Matter Repair after Ischemic Stroke through Osteopontin [Treg]

Project description:The precise mechanisms underlying the salutary effects of regulatory T cells (Tregs) on long-term tissue repair after stroke remain elusive. Here, , we performed RNAseq analysis on sorted CD4+CD25+Foxp3(GFP)+ Tregs from the ischemic brain and blood of DTR stroke mice 14d after surgery and from the blood of sham DTR mice. The unique patterns of gene expression of brain-infiltrating Tregs exhibited a significant enrichment for genes with functions in immunoregulation and cell-cell interactions, especially the activation of phagocytes.

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Transcriptional profiling of hair follicle stem cells in the presence and absence of Regulatory T-cells

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2017-04-28 | E-GEOD-76102 | biostudies-arrayexpress

Transcriptional profiling of Tregs and Teffs sorted from murine skin

Project description:The goal of this study was to identify transcripts that were preferentially expressed in skin-resident Tregs relative to skin-resident Teffs.

2018-12-02 | GSE121794 | GEO

Tumor invasion in draining lymph nodes is associated with Treg accumulation in breast cancer patients

Project description:Tumor-draining lymph node (TDLN) invasion by metastatic cells in breast cancer correlates with poor prognosis and is associated with local immunosuppression, which can be partly mediated by regulatory T cells (Tregs). Here, we study Tregs from matched tumor-invaded and non-invaded TDLNs, and breast tumors. We observe that Treg frequencies increase with nodal invasion, that Tregs express higher levels of co-inhibitory/stimulatory receptors than effector cells. Also, while Tregs show conserved suppressive function in TDLN and tumor, conventional T cells (Tconvs) in TDLNs proliferate and produce Th1-inflammatory cytokines, but are dysfunctional in the tumor. We describe a common transcriptomic signature shared by Tregs from tumors and nodes, including CD80, which is significantly associated with poor patient survival. TCR RNA-sequencing analysis indicates trafficking between TDLNs and tumors and ongoing Tconv/Treg conversion. Overall, TDLN Tregs are functional and express a distinct pattern of druggable co-receptors, highlighting their potential as targets for cancer immunotherapy.

2020-05-26 | E-MTAB-9112 | biostudies-arrayexpress

scRNA-seq analysis of dendritic cells in skin draining lymph nodes of CLEC4C-DTR mice treated with FITC hapten

Project description:Skin draining lymph node dendritic cell populations are known to be diverse and distinguishable via their migratory or resident properties as well as by lineage. We analyzed these populations at steady state and during the sensitization phase of the Th2-driven FITC-hapten contact hypersensitivity (CHS) model. We also analyzed the dendritic cells populations of CLEC4C-DTR+ mice treated with diptheria toxin, which demonstrate exacerbated pathology in the FITC-CHS model. We demonstrate the diversity of dendritic cell populations at steady state and during type 2 inflammation, and surprisingly, reveal a skewing of the DC populations in DT treated CLEC4C-DTR+ mice, with an enrichment of DC2 migratory populations and depletion of CD326+ migratory cells in addition to pDC.

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RNA-seq analysis of transcriptomes of Noc4L deficient and the Nco4L sufficient Treg cells

Project description:To gain in depth transcriptional information about the Noc4L deficient and Noc4L sufficient Tregs, we performed RNA-seq analysis to compare the transcriptomes of Noc4L deficient (CD4+GFP+CD62Lhigh, cKO) and Noc4L sufficient (CD4+GFP+CD62Lhigh, Het) Tregs activated in vitro with Dynabeads for 48 hours or 72 hours. We found ~25 unique genes with differential expression between the Noc4L deficient and Noc4L sufficient Treg cells when activated for 48 hours in vitro. And ~100 unique genes with differential expression between the Noc4L deficient and Noc4L sufficient Treg cells when activated for 72 hours in vitro in two times experiment.

2018-07-04 | GSE116572 | GEO

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