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MRNA expression during fracture healing in diabetic rats

ABSTRACT: The association between DM and impaired fracture healing including delayed union and nonunion has been documented in clinical and experimental settings. We examine mRNA expression specific for impaired fracture healing in diabetic rat.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE76364 | GEO | 2017/01/15

SECONDARY ACCESSION(S): PRJNA307105

REPOSITORIES: GEO

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miRNA expression during fracture healing in diabetic rats

Project description:The association between DM and impaired fracture healing including delayed union and nonunion has been documented in clinical and experimental settings. We examine miRNA expression specific for impaired fracture healing in diabetic rat.

2017-01-15 | GSE76365 | GEO

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Fracture healing in osteoporotic mice

Project description:Genome-wide comparative gene expression analysis of callus tissue of osteoporotic mice (Col1a1-Krm2 and Lrp5-/-) and wild-type were performed to identify candidate genes that might be responsible for the impaired fracture healing observed in Col1a1-Krm2 and Lrp5-/- mice. To investigate bone healing in osteoporosis, we performed fracture healing studies in wild-type mice (C57BL/6 genetic background) and the low bone mass strains Col1a1-Krm2 and Lrp5-/- (Schulze et al., 2010; Kato et al., 2002). Osteotomy was set in femora of female mice and stabilized by a semi-rigid fixator to allow fast bone healing (RM-CM-6ntgen et al., 2010). 21 days post surgery we analyzed the fracture calli by biochemical/histological methods, as well as micro-computed tomography, and observed impaired fracture healing in Col1a1-Krm2 and Lrp5-/- mice in comparison to wild-type. To identify genes that may be responsible for the impaired healing in osteoporotic mice, we performed microarray analysis of three independent callus samples of each genotype. The callus tissue was taken 10 days after surgery, because extensive bone formation took place at this point.

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Project description:The specific genes that distinguish normal fracture healing from abnormal healing or nonunion in humans are unknown. This study was an exploratory investigation of peripheral blood from acutely injured fracture patients collected over multiple days compared to age- and sex-matched healthy volunteers. Bone fragments were collected from acutely injured subjects during surgery through the Cooperative Human Tissue Network-Eastern Division (CHTN-ED http://pathology.med.upenn.edu/research/centers-and-institutes/cooperative-human-tissue-network). We used microarrays to do a global comparison between acutely injured (AI) subjects (AIS) and chronic Nonunion (NU) subjects vs. age- and sex-matched healthy volunteers (HV). We also compared tissue types by capturing bone fragments from the fracture site where possible.

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Project description:The specific genes that distinguish normal fracture healing from abnormal healing or nonunion in humans are unknown. This study explored global RNA expression in remnant bone fragments from acutely injured patients undergoing open primary fracture repair surgery. These data will be compared to blood samples collected from acutely injured subjects, chronic nonunion subjects, and a panel of healthy volunteers. We used microarrays to do a global comparison across bone fragments collected from different patients, and different bones. We also evaluated the role of remnant bone preparation which was either immediate freezing in liquid nitrogen or heat stabilized with the Denator Stabilizer(TM) system.

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