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Capture-C reveals preformed chromatin interactions between HIF-binding sites and distant promoters

ABSTRACT: Hypoxia inducible factor (HIF) directs an extensive transcriptional cascade that transduces numerous adaptive responses to hypoxia. Pan-genomic analyses, using chromatin immunoprecipitation and transcript profiling, have revealed large numbers of HIF-binding sites that are generally associated with hypoxia-inducible transcripts, even over long chromosomal distances. However, these studies do not define the specific targets of HIF-binding sites and do not reveal how induction of HIF affects chromatin conformation over distantly connected functional elements. To address these questions we deployed a recently developed chromosome conformation assay that enables simultaneous high-resolution analyses from multiple viewpoints. These assays defined specific long-range interactions between intergenic HIF-binding regions and one or more promoters of hypoxia-inducible genes, revealing the existence of multiple enhancer-promoter, promoter-enhancer and enhancer-enhancer interactions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE78100 | GEO | 2016/09/14

SECONDARY ACCESSION(S): PRJNA312614

REPOSITORIES: GEO

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Capture-C reveals preformed chromatin interactions between HIF-binding sites and distant promoters. [ChIP-Seq]

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