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Transcript profiling in splenic red pulp macrophages from SIRPa-KO mice and WT mice

ABSTRACT: To compare the splenic macrophages between SIRPα-knockout mice and WT mice, we performed a complete transcript profiling of the splenic red pulp macrophages from SIRPα-KO mice compared to WT mice using mRNA microarray as a discovery platform. SIRPα-KO mice and WT mice were kept under the same condition. Macrophages were isolated from spleen red pulp of SIRPα-KO mice and WT mice. RNA was then isolated from the same number of freshly isolated macrophages.

ORGANISM(S): Mus musculus

PROVIDER: GSE78191 | GEO | 2016/11/22

SECONDARY ACCESSION(S): PRJNA312895

REPOSITORIES: GEO

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Mus musculus

Project description:Transcript profiling in splenic red pulp macrophages from SIRPa-KO mice and WT mice

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Tissue printing: splenic red pulp macrophages of once-malaria infected mice are transcriptionally identical to prenatally seeded red pulp macrophages from uninfected mice.

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Project description:We processed RNA-sequencing on splenic CD11b+ macrophages isolated from 10-week old Mettl3f/f-LysM-Cre KO and littermate WT mice. NO_4-1, NO_4-2 are Mettl3f/f-LysM-Cre KO. NO_5-2, NO_5-3 are WT littermate controls.

2022-01-07 | GSE184318 | GEO

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Bone marrow derived macrophages RNA-Seq (WT vs SIRPa KO)

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Investigation of Bach2 function by heme-iron homeostasis in red-pulp macrophage.

Project description:Macrophages are central in regulating iron homeostasis. Transcription repressor Bach2 regulates by heme. Here we investigated the relationship between heme-regulated Bach2 and macrophage in spleen. We found that gene expression were not many change between WT and Bach2 knock out mice in red-pulp macrophage.Our results suggest that the function of the red-pulp macrophage is not dependent on according to expression of Bach2.

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