Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Temporal analysis of physiological and transcriptome responses during high light exposure that lead to dynamic acclimation in Arabidopsis

ABSTRACT: The photosynthetic capacity of mature leaves increases upon exposing plants to continuous or intermittent high light (HL). This is termed dynamic acclimation, which is an important determinant of plant productivity. We hypothesized that genes coding for transcription regulators (TRs), which respond rapidly (≤1h) and transiently to HL, initiate dynamic acclimation. To find such genes, a highly replicated time series transcriptomic dataset was generated from leaf 7 of low light (LL)-grown Arabidopsis thaliana plants subjected to HL for up to 6h. This revealed 3844 HL-responsive differentially expressed genes (DEGs). 49 transiently HL-induced TR DEGs were selected for Bayesian modeling, which revealed that B-BOX TRANSCRIPTION (CO) FACTOR32 (BBX32) was the most connected gene in an inferred network. Dynamic acclimation was elicited in Arabidopsis Col-0 by exposure to daily 4h HL over 5 days. Using this procedure on over-expressing (OE) and null mutant plants, it was shown that BBX32 is a negative regulator of dynamic acclimation. The same modeling connected BBX32 to the TR gene LONG HYPOCOTYL5 (HY5), but unlike BBX32, HY5 was shown to positively regulate dynamic acclimation. The severely diminished response to daily HL of BBX32–OE/hy5 plants suggest that in wild type plants, BBX32 and HY5 exert a balanced, flexible and complete regulation of dynamic acclimation.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE78251 | GEO | 2018/08/03

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The impact of the genes BBX32 and HY5 on the high light - responsive transcriptome of Arabidopsis thaliana

Project description:Exposure of mature fully expanded leaves of Arabidopsis to a 7.5 fold increased light intensity above growth light conditions (high light; HL) tirggers stress defensive responses but also initiates cellular processes, that if such conditions persist, can lead to increased photosynthetic capacity. This process is called dynamic acclimation. By using variational Bayesian state space modelling on eariler GEO deposited time series HL data (see GSE78251) a gene regulatory network of (co) transcription factor genes was inferred. The most connected gene in this network is BBX32, which was subequently shown to be a negative regulator of dynamic acclimation. Also present in the inferred network is HY5, which is known from studies on seedling photomorphogenesis to be antagonistic in its action to BBX32. Subsequently, it was demonstrated that HY5 is indeed a positive regulator of dynamic acclimation. This RNAseq-based study seeks to provide gene expression data that will help to link the immediate impact of these genes on the HL transcriptome to the longer term (several days) physiological manifestation of dynamic acclimation.

2021-04-01 | GSE158898 | GEO

High Light and High Temperature Reduce Photosynthetic Efficiency via Different Mechanisms in the C4 Model Setaria viridis

Project description:C4 plants frequently experience damaging high light (HL) and high temperature (HT) conditions in native environments, which reduce growth and yield. However, the mechanisms underlying these stress responses in C4 plants have been under-explored, especially the coordination between mesophyll (M) and bundle sheath (BS) cells. We investigated how the C4 model plant Setaria viridis responded to a four-hour HL or HT treatment at the photosynthetic, transcriptomic, and ultrastructural levels. Although we observed a comparable reduction of photosynthetic efficiency in HL- or HT-treated leaves, detailed analysis of multi-level responses revealed important differences in key pathways and M/BS specificity responding to HL and HT. We provide a systematic analysis of HL and HT responses in S. viridis, reveal different acclimation strategies to these two stresses in C4 plants, discover unique light/temperature responses in C4 plants in comparison to C3 plants, and identify potential targets to improve abiotic stress tolerance in C4 crops.

2021-08-01 | GSE178320 | GEO

Gene expression profile of T-DM1 resistant and parental cell lines

Project description:The esophageal cancer cell line OE-19 was used to select resistant cells to T-DM1. They were selected by prolonged exposure to increasing concentrations of T-DM1 in the absence or presence of ciclosporin A (CsA). Two cell lines resulted from these experimental conditions: OE-19 TR in the absence of CsA and OE-19 TCR in the presence of CsA. The parental cell line was named OE-19 S. The present study aims to detect the transcriptomic changes between OE-19 S, OE-19 TR and OE-19 TCR cell lines to identify the deregulated pathways involved in acquired resistance to T-DM1.

2017-01-11 | GSE93352 | GEO

Global transcriptome of Arabidopsis plants (Col-0, Rld2 and Shahdara genotypes) inoculated by the fungal pathogen Sclerotinia sclerotiorum following temperate, mediterranean and continental acclimation

Project description:The short-term, reversible process that allows plants to cope with immediate environmental fluctuations is often referred to as acclimation. Plant acclimation help maintain the balance of natural systems, supporting biodiversity and the services that ecosystems provide, such as carbon sequestration and water regulation. With climate change modifying the distribution area of plants and causing more frequent and severe weather events, knowledge of how plants acclimate can inform strategies to manage ecosystems and agriculture. Analyses of plant immune responses under abiotic constraints generally focus on pathogen inoculation under prolonged and stable abiotic conditions. Daily fluctuations of the environment may alter plant metabolism, growth and flowering as well as gene regulation and invasive growth of fungal pathogens. Yet, how plant immunity acclimates to daily temperature fluctuations remains largely unexplored. To study the molecular bases of quantitative disease resistance acclimation, we performed a global transcriptome analysis of Arabidopsis thaliana accessions Col-0, Rld-2 and Sha grown in temperate, continental and Mediterranean climates, followed or not by Sclerotinia sclerotiorum inoculation.

2024-10-24 | GSE272240 | GEO

Acclimation of photosynthesis to changes in the environment results decreases oxidative stress in Arabidopsis thaliana

Project description:Dynamic acclimation of photosynthesis plays an important role in increasing plant fitness under variable light environments. Acclimation is mediated by a glucose-6-phosphate/phosphate translocator, GPT2. This study examined whether plants lacking GPT2, which are defective in acclimation to increases in light, are more susceptible to oxidative stress. To understand this, we used the model plant of Arabidopsis thaliana (accession Wassilewskija-4 (Ws-4)) and compared this to mutants lacking GPT2. Plants were grown at low light (100 μmol m−2 s−1) for 7 weeks. For acclimation experiments, a set of plant from low light was transferred to 400 μmol m−2 s−1 for 7 days. Microarray analysis showed that gpt2 plants showed a greater induction of stress related genes relative to WT.

2021-03-24 | E-MTAB-10282 | biostudies-arrayexpress

Analysis of reversibility of transcriptome responses to abiotic stress conditions in Arabidopsis thaliana

Project description:Plants acclimate to environmental fluctuations by transitory reconfigurations the homeostatic network. Primary studies suggested that transcriptome responses to deal with fluctuations in light intensity and temperature tend to reversibility after stress removal in the model plant Arabidopsis thaliana. To gain more insight into this pattern in the context of acclimation, RNA-Seq analysis were conducted in Arabidopsis thaliana after different abiotic stress treatments consisting in high light (HL), high humidity, drought, heat, cold and combinations among factors or after recovery periods. Our transcriptome study is in line of a general pattern wherby transcriptome changes in response to adverse environments are prone to return to the basal state during the de-acclimation phase.

2023-06-28 | GSE226105 | GEO

A light signaling factor, ELONGATED HYPOCOTYL 5 (HY5) interacts with Histone deacetylase 9 (HDA9) to play a suppressive role in the glucosinolate biosynthesis in Arabidopsis

Project description:In this study, we found that a light signaling factor, Long Hypocotyl 5 (HY5) is closely involved in the regulation of GSLs contents in light condition. In addition, HY5 was shown to physically interact with a histone deacetylase HDA9 and bind to proximal promoter region of MYB29 and IMD1 to suppress aliphatic GSL biosynthetic process. These results demonstrate that HY5 acts to suppress GSL accumulation at daytime, thus properly modulating the GSL contents on a daily basis of Arabidopsis plant.

2024-06-12 | GSE250418 | GEO

Identification of mouse lncRNA ENSMUST00000219456(GM32255) interacting proteins in HL-1 cardiomyocytes by pull-down assays coupled to MS

Project description:In this study, pull down assays combined with mass spectrometry analysis were performed in HL-1 cardiomyocytes transfected with control plasmid (OE-Vector) or LOC107984012-overexpression plasmid to identify the repertoire of LOC107984012 interacting proteins.

2024-01-26 | PXD036966 | Pride

Genome-wide gene expression analysis reveals a critical role for CRY1 in the Response of Arabidopsis to High Irradiance

Project description:Exposure to high irradiance results in dramatic changes in nuclear gene expression in plants. However, little is known about the mechanisms by which changes in irradiance are sensed and how the information is transduced to the nucleus to initiate the genetic response. To investigate whether the photoreceptors are involved in the response to high irradiance, we analyzed expression of ELIP1, ELIP2, APX2 and LHCB2.4 in the phyA, phyB, cry1 and cry2 photoreceptor mutants and hy5 and hyh transcription factor mutants. Following exposure to high intensity white light for 3 h (HL, 1000 micro mol quanta m-2 s-1) expression of ELIP1/2 and APX2 was strongly induced and LHCB2.4 expression repressed in wild type. The cry1 and hy5 mutants showed specific mis-regulation of ELIP1/2 and we show that the induction of ELIP1/2 expression is mediated via CRY1 in a blue light intensity-dependent manner. Furthermore, using the Affymetrix Arabidopsis 24K Gene-Chip we showed that 77 of the HL responsive genes are regulated via CRY1, and 26 of those genes were also HY5 dependent. As a consequence of the mis-regulation of these genes the cry1 mutant displayed a high irradiance-sensitive phenotype with significant photoinactivation of PSII, indicated by reduced Fv/Fm. Thus, we describe a novel function of CRY1 in mediating plant responses to high irradiances that is essential to the induction of photoprotective mechanisms. This indicates that high irradiance can be sensed in a chloroplast-independent manner by a cytosolic/nucleic component. Keywords: photoreceptor, transcription factor, cytosolic/nuclear component, chloroplast, stress response, photoprotection

2007-05-09 | GSE7743 | GEO

Identification of A Novel bZIP Transcription Factor in Camellia sinensis as A Negative Regulator of Freezing Tolerance in Transgenic Arabidopsis

Project description:Basic region/leucine zipper (bZIP) transcription factors play vital roles in the abiotic stress response of plants. However, little is known about the function of bZIP genes in Camellia sinensis. Here, we show that CsbZIP6 is induced during cold acclimation in tea plant. Constitutive overexpression of CsbZIP6 in Arabidopsis lowered the plants’ tolerance to freezing stress and ABA exposure during seedling growth. Compared to wildtype (WT) plants, CsbZIP6 overexpression (OE) lines exhibited increased levels of electrolyte leakage (EL) and malondialdehyde (MDA) contents and reduced levels of total soluble sugars (TSS) under cold stress conditions. Microarray analysis of transgenic Arabidopsis revealed that many differentially expressed genes (DEGs) between OE lines and WT plants could be mapped to ‘response to cold’ and ‘response to water deprivation’ terms based on GO analysis. Interestingly, CsbZIP6 overexpression repressed most of the cold- and drought-responsive genes as well as the starch metabolism under cold stress conditions. Taken together, our data suggests that CsbZIP6 functions as a negative regulator of the cold stress response in Arabidopsis thaliana, potentially by down-regulating cold-responsive genes. To obtain insights into the molecular mechanisms by which CsbZIP6 mediates senstivity to cold stress in Arabidopsis plants, gene expression profiles in leaves of two CsbZIP6 OE lines and WT plants under normal (22ºC) and cold (4ºC) conditions were compared. The Agilent Arabidopsis Gene Expression (4×44K, Design ID: 021169) was used in this experiment.

2017-07-01 | GSE84570 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data