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Whole translatome profiling of Human Hepatoma culture Huh7.5 and Huh7.5M

ABSTRACT: Huh7.5 cells, when grown in presence of 1-2mg/ml of Geneticin, generarated colonies with cells that were morphologically distinct from the parental cells. These cells were substantially smaller in size, had spindle like features and grew faster. They expressed mesenchymal markers and were named as Huh7.5M cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE79464 | GEO | 2017/02/01

SECONDARY ACCESSION(S): PRJNA315883

REPOSITORIES: GEO

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Whole transcriptome profiling of Human Hepatoma culture Huh7.5 and Huh7.5M

Project description:Huh7.5 cells, when grown in presence of 1-2mg/ml of Geneticin, generarated colonies with cells that were morphologically distinct from the parental cells. These cells were substantially smaller in size, had spindle like features and grew faster. They expressed mesenchymal markers and were named as Huh7.5M cells.

2017-02-01 | GSE78085 | GEO

Control Huh7.5 cell vs HPI cell (HCV-persistently-infected Huh7.5 cell)

Project description:Transcriptional profiling of human hepatoma cell lines comparing control uninfected Huh7.5 cells with Huh7.5 cells persistently infected with HCV (HPI cell). The latter maintains and produces HCV.

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Culturing cells in human serum: differentiation and metabolic reprogramming in human hepatoma cells

Project description:Huh7.5 cells, which are cultured in human serum containing media instead of FBS containing media, undergo growth arrest and become differentiated. In this study we further characterized human serum cultured Huh7.5 cells, particularly with regards to morphology, metabolic profile and hepatocyte specific functions. Similar to primary hepatocytes, HS-cultured Huh7.5 cells become columnar, are polarized, and develop bile canalicular structures on the apical side of the cells. Further analysis by microarray showed that significant transcriptional changes occur in 22-32% of the genes. HS cultured Huh7.5 cells undergo extensive metabolic re-programming. We showed a reversal of a Warburg-like metabolic profile that is typical for proliferating cells. In HS cultured cells large lipid droplets can be found in addition to large glycogen stores. Metabolic modeling shows an increased reliance on beta-oxidation, which was confirmed by an increase in ketone body production, the end products of β-oxidation. Additionally we showed increased conversion of glucose to glycogen. Bile secretion is increased, as are the enzymes involved in the degradation of xenobiotics. Overall these data show that only changing the serum in cell culture media can result in the establishment of a cell type that morphologically, metabolically and functionally resemble primary hepatocytes.

2017-10-01 | GSE87684 | GEO

Polysome profiling of Huh7.5 cells expressing HCV NS5A

Project description:Hepatitis C Virus protein NS5A was found to upregulate assembly of cap binding initiation complex eIF4F in Huh7.5 cells. NS5A also was found to associate with translation machinery. To understand consequences of NS5A mediation in host translation, we analyzed mRNA associated with polysome fractions of NS5A expressing Huh7.5 cells and compared them with the corresponding fractions from control cells.

2014-05-01 | GSE46273 | GEO

Polysome profiling of Huh7.5 cells expressing HCV NS5A

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Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-7 mimic

Project description:Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-7 mimic

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Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-124 mimic

Project description:Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-124 mimic

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Transciptomic analysis in HAV infected Huh7.5 cells

Project description:Transciptomic analysis in HAV infected Huh7.5 cells

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Homo sapiens isolate:Huh7.5

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Exosome-mediated transmission of hepatitis C virus between human hepatoma Huh7.5 cells.

Project description:Recent evidence indicates there is a role for small membrane vesicles, including exosomes, as vehicles for intercellular communication. Exosomes secreted by most cell types can mediate transfer of proteins, mRNAs, and microRNAs, but their role in the transmission of infectious agents is less established. Recent studies have shown that hepatocyte-derived exosomes containing hepatitis C virus (HCV) RNA can activate innate immune cells, but the role of exosomes in the transmission of HCV between hepatocytes remains unknown. In this study, we investigated whether exosomes transfer HCV in the presence of neutralizing antibodies. Purified exosomes isolated from HCV-infected human hepatoma Huh7.5.1 cells were shown to contain full-length viral RNA, viral protein, and particles, as determined by RT-PCR, mass spectrometry, and transmission electron microscopy. Exosomes from HCV-infected cells were capable of transmitting infection to naive human hepatoma Huh7.5.1 cells and establishing a productive infection. Even with subgenomic replicons, lacking structural viral proteins, exosome-mediated transmission of HCV RNA was observed. Treatment with patient-derived IgGs showed a variable degree of neutralization of exosome-mediated infection compared with free virus. In conclusion, this study showed that hepatic exosomes can transmit productive HCV infection in vitro and are partially resistant to antibody neutralization. This discovery sheds light on neutralizing antibodies resistant to HCV transmission by exosomes as a potential immune evasion mechanism.

| S-EPMC3740869 | biostudies-literature

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