Project description:Our work demonstrated that PPAR-α regulates miR-7, a microRNA which regulates SREBP1 signaling. We used microarrays to detail the effect of miR-7 on hepatic gene expression, and, within activated genes, identified an enrichment for genes with SREBP1 binding sites in their promoters.
Project description:Our work demonstrated that 25-hydroxycholesterol induces the expression of miR-185, a microRNA which reinforces the oxysterol's antiviral effects on hepatic metabolism. We used microarrays to detail the effect of miR-185 on hepatic gene expression, and, within repressed genes, identified an enrichment for genes associated with cholesterol biosynthesis.
Project description:Our work demonstrated that miR-124 regulates fatty acid and triglyceride homeostasis. We used microarrays to detail the effect of miR-124 on hepatic gene expression, and, within downregulated genes, identified an enrichment for genes involved in lipid metabolism.
Project description:Transcriptional profiling of human hepatoma cell lines comparing control uninfected Huh7.5 cells with Huh7.5 cells persistently infected with HCV (HPI cell). The latter maintains and produces HCV.
Project description:Huh7.5 cells, when grown in presence of 1-2mg/ml of Geneticin, generarated colonies with cells that were morphologically distinct from the parental cells. These cells were substantially smaller in size, had spindle like features and grew faster. They expressed mesenchymal markers and were named as Huh7.5M cells.
Project description:Huh7.5 cells, when grown in presence of 1-2mg/ml of Geneticin, generarated colonies with cells that were morphologically distinct from the parental cells. These cells were substantially smaller in size, had spindle like features and grew faster. They expressed mesenchymal markers and were named as Huh7.5M cells.
Project description:A microarray experiment was performed to investigate changes in gene expression induced in the Huh7.5 hepatoma cell line following treatment for 24 hours with recombinant human Bone Morphogenetic Protein 6 (BMP6) (18 nM).
Project description:KYSE510 cells were treated with 100 nM PAF for 24 hours,and then KYSE510 cells were collected for gene expression analysis using Agilent SurePrint G3 Human Gene Expression v3 (8×60K) Microarray. Investigation of the gene network of KYSE510 cells regulated by PAF.