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Liz transcription is required for evicting H3K27me3 marks at the Gpr1/Zdbf2 locus through the deposition of DNA methylation marks.

ABSTRACT: To study the role of the Liz transcript on local chromatin features during early development, we used a culture-based system. Mouse embryonic stem cells (ESCs) with a hypomethylated genome were obtained through their culture in 2i medium supplemented with vitamin C. To recapitulate de novo DNA methylation, these cells were directed towards an epiblast-like cell fate (EpiLC) during seven days of culture in Activin A and Fgf2. The effect of Liz transcription on the maintenance of H3K27me3 marks at the Gpr1/Zdbf2 locus was inferred by the comparative ChIP-seq analysis of H3K27me3 enrichment in WT (E14) cells versus ∆Liz cells- which carry a TALEN-engineered 1.7kb homozygous deletion of the Liz Transcription Start Size (TSS)- at Day 0 (D0), D4 and D7 of EpiLC differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE79549 | GEO | 2016/11/01

SECONDARY ACCESSION(S): PRJNA316144

REPOSITORIES: GEO

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