Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The lncRNA microarray analysis of 16HBE cell treated with PM2.5 samples in Guangzhou

ABSTRACT: In order to assess the alteration of lncRNA expression in 16HBE cell treated with PM2.5 samples, we determined the lncRNA expression profiles in 16HBE cell treated with PBS (control group) and PM2.5 samples (low dose 125 μg/mL and high dose 500 μg/mL) using lncRNA Microarray.

ORGANISM(S): Homo sapiens

PROVIDER: GSE79570 | GEO | 2016/03/25

SECONDARY ACCESSION(S): PRJNA316200

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The lncRNA microarray analysis of 16HBE cell treated with PM2.5 samples in Guangzhou

Project description:In order to assess the alteration of lncRNA expression in 16HBE cell treated with PM2.5 samples, we determined the lncRNA expression profiles in 16HBE cell treated with PBS (control group) and PM2.5 samples (low dose 125 Î¼g/mL and high dose 500 Î¼g/mL) using lncRNA Microarray. 16HBE Cells were treated with PM2.5 suspension at concentration of 125 Î¼g/mL and 500 Î¼g/mL, and PBS was used in the control group for 48 h. Then, total RNAs were extracted for lncRNA chip preparation and analysis.

2016-03-25 | E-GEOD-79570 | biostudies-arrayexpress

Sequencing of human chain specific ribosome transcriptional group

Project description:The purpose of this study is to investigate whether the expression profile of long chain non coding RNA (lncRNA) in different concentrations of PM2.5 after treatment of normal human bronchial cells is changed. The total RNAs were extracted from 16HBE cells after exposure to either PM2.5, at concentration of 50 μg/mL and 100μg/mL, or PBS (control group) for 48h. lncRNA-sequencing, detection and analysis was performed. Fold change ≥ 2 and p ≤ 0.05 were used as a cutoff values to determine significant differential expression Further, cluster analyses were performed to classify lncRNAs based on their distinguishable expression in different samples.

2018-04-13 | GSE113071 | GEO

RNA-seq analysis identifies the effect of PM2.5 on human nasal epithelial cells cultured under air-liquid interface (ALI) conditions until differentiated.

Project description:In this study, we performed high-throughput sequencing to evaluate the gene expression profiles of human nasal epithelial cells (HNECs) cultured under air-liquid interface (ALI) conditions until differentiated and then stimulated with PM2.5 (100 μg/ml) for 24 hours.

2023-09-25 | GSE243618 | GEO

Homo sapiens

Project description:The lncRNA microarray analysis of 16HBE cell treated with PM2.5 samples in Guangzhou

| PRJNA316200 | ENA

Genome-wide analysis reveals mucociliary remodeling of the airway epithelium induced by PM2.5

Project description:Air pollution particulate matter <2.5 microns (PM2.5) is associated with poor respiratory outcomes. Mechanisms underlying PM2.5-induced lung pathobiology are poorly understood, but likely involve cellular and molecular changes to the airway epithelium. We extracted and chemically characterized the organic and water-soluble components of contemporary ambient air pollution PM2.5 samples. We then determined the whole transcriptome responses of human mucociliary airway epithelial cultures (n=12) to a dose series of PM2.5 extracts. We found PM2.5 organic, but not water-soluble, constituents elicited a potent, dose-dependent transcriptomic response from the mucociliary epithelium. Epithelial exposure to a moderate organic extract (OE) dose modified the expression of 424 genes, which included activation of aryl-hydrocarbon receptor (AHR) signaling and an interleukin-1 inflammatory program. We generated an OE response network composed of 8 metagroups, which exhibited high connectivity through the CYP1A1, IL1A, and IL1B genes. This OE exposure also robustly activated a mucus secretory expression program (>100 genes), which included transcriptional drivers of mucus metaplasia (SPDEF, FOXA3). Exposure to a higher OE dose modified the expression of 1,240 genes and further exacerbated expression responses observed at the moderate dose, including the mucus secretory program. Moreover, the higher OE dose significantly increased the MUC5AC/MUC5B gel-forming mucin expression ratio and strongly downregulated ciliated cell expression programs, including key ciliating cell transcription factors (FOXJ1, MCIDAS). Our results suggest organic chemicals in PM2.5 likely drive cellular remodeling of the airway epithelium, punctuated by mucus metaplasia and loss of ciliated cells. This epithelial remodeling may underlie poor respiratory outcomes associated with high PM2.5 exposure.

2020-04-10 | GSE144770 | GEO

Airborne PM2.5 samples

Project description:Airborne PM2.5 samples Metagenome

| PRJNA810770 | ENA

Wistar rat model of COPD: Cigarette smoking exposure and fine particulate matter (PM2.5) exposure vs. control.

Project description:Genome-wide analysis of lncRNA expression profiles in COPD rat model exposed by cigarette smoking (CS) and fine particulate matter (PM2.5). Goal was to explore the differences and similarities lncRNAs expression in rats model of COPD exposed by CS and PM2.5.

2021-06-21 | GSE178513 | GEO

mRNA expression profile of mouse CD4 T cells (treated vs. untreated with PM2.5 suspension)

Project description:Purpose: PM2.5 can impact mouse TH17 cell differentitation. To Investigator the genes altered by PM2.5 during TH17 differentiation, mRNA-seq was performed. Methods: Naïve T cells were isolated from mouse spleen by magnetic beads. These naïve T cells were stimulated by CD3/CD28 antibodies and cultured under TH17 polarization conditions for 4 days before harvested. For PM2.5 group, 100ug/ml of PM2.5 suspensions were added to culture medium 6 hours after stimulation. The total RNA was extracted using the TRIzol reagent (Invitrogen). The library construction and sequencing was performed by Illumina Xten at Shanghai Biotechnology Corporation.

2019-07-01 | GSE124660 | GEO

Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

Project description:Deinococcus radiodurans R1 Cells was treated with MMC (5 μg/ml) : Control treated with MMC (5 μg/ml) vs. bphPR deletion mutant treated with MMC (5 μg/ml)

| PRJNA254487 | ENA

Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells

Project description:To identify Ras, MEK and ERK target genes which regulate tight junction formation in 16HBE bronchial epithelial cells

2018-12-01 | GSE63229 | GEO