Project description:HEK293 cells were heatshocked and differentially expressed transcripts were identified Transcriptomes of heatshocked HEK293 cells were compared to control cells. Heatshock and control samples were treated and sequenced in triplicate.
Project description:Comparison of gene expression profile of HEK293-CT cells and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1) We used micrarrays to identify the repertoire of genes differentially expressed upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.
Project description:To get insight of molecular mechanism of GPR39, we investigated transcriptomic response in GPR39 overexpressed HEK293 cells after TC-G-1008 stimulation We identified 814 differentially expressed genes (DEGs) fold change cutoff = 1.5 in the GPR39 overexpressed HEK293 and GPR39 overexpressed HEK293 cells with TC-G-1008 treatment, those including 364 up-regulated and 450 down-regulated genes These findings suggest that MAPK/Erk, PI3K/Akt and Glycerolipid metabolism signaling pathways would be putative signaling pathways dominantly altered by GPR39 activation.
Project description:Poly-A selected mRNA-seq was performed on HEK293 cells stably expressing either shRNA targeting STUB1 (shSTUB1 / shCHIP) or non-targeting control shRNA (shCTL). Biological triplicates were collected under basal conditions (37°C) and after acute heat shock (42°C for 30 minutes) to investigate the role of the STUB1/CHIP co-chaperone/E3 ligase in the cellular heat-shock response and proteostasis. This dataset supports the RNA-seq component of the study examining biochemical and clinical implications of STUB1 mutations in spinocerebellar ataxia type 48 (SCA48).
Project description:we report the partial methylome (CG-rich regions) of HEK293 cells and HEK293 cells over-expressing the BAHD1 gene (HEK-BAHD1) We used MEDIP-seq to identify genomic regions differentially methylated upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.
Project description:Comparison of methylome of HEK293-CT cells and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1) We used BS-seq to identify genomic regions differentially methylated upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.