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Comparison between WT and apl in a novel in vitro tissue culture system, VISUAL

ABSTRACT: We established a novel in vitro tissue culture system (named VISUAL), in which xylem and phloem differentiation can be induced with Arabidopsis thaliana cotyledons To compare gene expression profiles between WT and apl during vascular development, we performed GeneChip analysis using VISUAL.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE80026 | GEO | 2016/05/25

SECONDARY ACCESSION(S): PRJNA317646

REPOSITORIES: GEO

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Project description:We profiled transcripts from sorted phloem cells of wild-type and apl mutants to identify the genes regulated by APL in phloem. We profiled transcripts from sorted phloem cells of wild-type and apl mutants to identify the genes regulated by APL in phloem. Three biological replicates for control WT and apl mutants were used in thes study. Root tissue was protoplasted and sorted using fluorescence-activated cell sorting (FACS). Total RNA was extracted from GFP+ protoplasts.

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