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Protein Expression in TW Formation

ABSTRACT: Tangential sections of 30 µm thickness were taken across the secondary phloem and developing secondary xylem, sections were then pooled to represent the tissues from specific developmental stages. Phloem (P) comprises 3 sections, the cambium (C) - 2 sections, the radial expansion zone (E) - 4 sections, the first to third xylem development zones (X1-X3) - 3 sections each, and the forth to sixth xylem development zones (X4-X6) - 6 sections each.

INSTRUMENT(S): Synapt G2 HDMS

ORGANISM(S): Populus Tremula

TISSUE(S): Wood

SUBMITTER: Joakim Bygdell

LAB HEAD: Gunnar Wingsle

PROVIDER: PXD005715 | Pride | 2017-06-23

REPOSITORIES: Pride

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			Action	DRS
	2014-01-20_TW_Mem_01.raw.tgz	Raw
	2014-01-20_TW_Mem_02.raw.tgz	Raw
	2014-01-20_TW_Mem_03.raw.tgz	Raw
	2014-01-20_TW_Mem_04.raw.tgz	Raw
	2014-01-20_TW_Mem_05.raw.tgz	Raw

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Publications

Protein expression in tension wood formation monitored at high tissue resolution in Populus.

Bygdell Joakim J Srivastava Vaibhav V Obudulu Ogonna O Srivastava Manoj K MK Nilsson Robert R Sundberg Björn B Trygg Johan J Mellerowicz Ewa J EJ Wingsle Gunnar G

Journal of experimental botany 20170601 13

Tension wood (TW) is a specialized tissue with contractile properties that is formed by the vascular cambium in response to gravitational stimuli. We quantitatively analysed the proteomes of Populus tremula cambium and its xylem cell derivatives in stems forming normal wood (NW) and TW to reveal the mechanisms underlying TW formation. Phloem-, cambium-, and wood-forming tissues were sampled by tangential cryosectioning and pooled into nine independent samples. The proteomes of TW and NW samples ...[more]

PMID: 28633298

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Project description:Regeneration is a common strategy for plants to repair their damaged body plans after attack from other organisms or physical assaults. Trees with bark girdling on a large scale will grow new bark within one month and this bark regeneration after girdling system has been proven to be an efficient method to study secondary vascular development as well as plant tissue regeneration in vivo. We herein show the molecular features of differentiating xylem cell fate switch process during secondary vascular tissue (SVT) regeneration in Populus. Based on our data, we propose a working model to illustrate the molecular dynamics underlying xylem cell fate switch process during SVT regeneration, which is significant to understand the pattern formation during the SVTs regeneration and also would shed light on the mechanisms of tissue regeneration in plants. Specific regenerated tissues of Populus at different stages were isolated by tangential cryo-sectioning. Total RNA from cryo-sections representing different regenerating tissues was extracted for Affymetrix Poplar Whole Genome Array hybridization. Five samples (two replicates for each sample) were used for gene expression analysis: differentiating xylem (diX, Stage 0), dedifferentiating xylem cells (deX, Stage I), regenerated phloem (rPh, Stage II), differentiating regenerated cambium (diC, Stage II) and regenerated cambium (rC, Stage III). In addition, one pooled genomic DNA sample from cryo-sections of differentiating xylem from two trees was isolated for DNA hybridization to produce a new CDF file that was used to mask out some potentially cross-hybridizing probesets from the standard Affymetrix Poplar Genome Array. Supplementary file: poplar.cdf

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