Genomics

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PfAP2-I-GFP anti-GFP and anti-IgG ChIP-seq


ABSTRACT: To determine the genome-wide occupancy of the Plasmodium falciparum transcriptional regulator of invasion PfAP2-I (PfDd2_100013100/PF3D7_1007700), we used chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq). Synchronized, schizont stage, 40 hours post-invasion, cultures of parasites expressing the AP2-I-GFP fusion protein were treated with formaldehyde to crosslink proteins to DNA and harvested. After shearing the DNA, the chromatin was incubated with anti-GFP antibody or IgG (as control) for immunoprecipitation. This material was used to generate Illumina sequencing libraries. The final libraries were multiplexed with fourteen barcoded samples per lane on an Illumina HiSeq 2500 system to generate 150 base pair single-end reads.

ORGANISM(S): Plasmodium falciparum

PROVIDER: GSE80293 | GEO | 2017/06/01

SECONDARY ACCESSION(S): PRJNA318518

REPOSITORIES: GEO

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