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MF59 and Pam3CSK4 boost adaptive responses to influenza subunit vaccine through an IFN type I independent mechanism of action

ABSTRACT: Analysis of whole mouse muscle and inguinal lymph node gene expression signature induced after 6h by in-vivo intramuscularly administration of MF59, alum, CpG, resiquimod (R848), Pam3CSK4 and DMSO and PBS controls. Analysis of splenocyte gene expression signature induced by the same treatments after 6h of incubation. MF59 and alum are licensed human vaccine adjuvants; CpG is a TLR9-agonist adjuvant; resiquimod (R848) is a TLR7/8-agonist adjuvant and Pam3CSK4 is a TLR2-agonist adjuvant.

ORGANISM(S): Mus musculus

SUBMITTER: Elaine Tritto

PROVIDER: E-MTAB-942 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MF59 and Pam3CSK4 boost adaptive responses to influenza subunit vaccine through an IFN type I-independent mechanism of action.

Caproni Elena E Tritto Elaine E Cortese Mario M Muzzi Alessandro A Mosca Flaviana F Monaci Elisabetta E Baudner Barbara B Seubert Anja A De Gregorio Ennio E

Journal of immunology (Baltimore, Md. : 1950) 20120220 7

The innate immune pathways induced by adjuvants required to increase adaptive responses to influenza subunit vaccines are not well characterized. We profiled different TLR-independent (MF59 and alum) and TLR-dependent (CpG, resiquimod, and Pam3CSK4) adjuvants for the ability to increase the immunogenicity to a trivalent influenza seasonal subunit vaccine and to tetanus toxoid (TT) in mouse. Although all adjuvants boosted the Ab responses to TT, only MF59 and Pam3CSK4 were able to enhance hemaggl ...[more]

PMID: 22351935

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