Transcriptomics

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Gene expression signatures for B cells from tumor draining lymph node and normal lymph node


ABSTRACT: To determine the different gene signatures between B lymphocytes from tumor draining lymph node (DLN) and normal lymph node (NLN), we have employed gene microarray as a discovery platform to identify gene signatures of tumor-educated B cells in DLN from tumor-bearing mice, taking NLN from normal mice as a control. We subcutaneously inoculated Balb/c mice with breast cancer cell line 4T1. Two weeks later, DLN was harvest and B cells were purified as descript in “treatment protocol”. From gene microarray, we found that B cells in DLN showed quite different transcript profiles from that in NLN.

ORGANISM(S): Mus musculus

PROVIDER: GSE81146 | GEO | 2018/05/01

REPOSITORIES: GEO

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