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A transcript profile of Medicago truncatula 2HA tissue culture with auxin treatment

ABSTRACT: Leaf explants of the superembryogenic Medicago truncatula line 2HA were treated with auxin (1-naphthaleneacetic acid) for one week to induce the formation of roots (Imin et al J Exp Bot 58:439-451). Gene expression in the leaves and the NAA treated tissue cultures was compared to identify transcripts expressed during the commitment to root formation in tissue culture. We have used the Affymetrix Medicago Genome Array GeneChip to compared gene expression in Medicago truncatula leaves and leaf explants that have been cultured for one week on NAA, to identify genes expressed during the commitment to root formation in tissue culture. Keywords: Cell type comparison

ORGANISM(S): Medicago sativa Sinorhizobium meliloti Medicago truncatula

PROVIDER: GSE8138 | GEO | 2007/10/01

SECONDARY ACCESSION(S): PRJNA101025

REPOSITORIES: GEO

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Transcription profiling of Medicago truncatula 2HA tissue culture with auxin treatment

Project description:Leaf explants of the superembryogenic Medicago truncatula line 2HA were treated with auxin (1-naphthaleneacetic acid) for one week to induce the formation of roots (Imin et al J Exp Bot 58:439-451). Gene expression in the leaves and the NAA treated tissue cultures was compared to identify transcripts expressed during the commitment to root formation in tissue culture. We have used the Affymetrix Medicago Genome Array GeneChip to compared gene expression in Medicago truncatula leaves and leaf explants that have been cultured for one week on NAA, to identify genes expressed during the commitment to root formation in tissue culture. Experiment Overall Design: Medicago truncatula 2HA leaves and leaf explants treated with NAA for one week were collected; total RNA was extracted and used for hybridization to Affymetrix arrays

2008-06-17 | E-GEOD-8138 | biostudies-arrayexpress

A transcript profile of Medicago truncatula 2HA and Jemmalong during somatic embryogenesis

Project description:The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared transcriptomes of tissue cultures from leaf explants of these two lines. We have used the Affymetrix Medicago Genome Array GeneChip to compare leaf explants of Medicago truncatula Jemalong and the superembryogenic line 2HA after two weeks of tissue culture on 10 µM 1-naphthaleneacetic acid (NAA) and 4 µM 6-benzylaminopurine (BAP) (P4 10:4) Keywords: Genotype comparison

2007-09-01 | GSE8131 | GEO

Transcription profiling of Medicago truncatula 2HA and Jemmalong during somatic embryogenesis

Project description:The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared transcriptomes of tissue cultures from leaf explants of these two lines. We have used the Affymetrix Medicago Genome Array GeneChip to compare leaf explants of Medicago truncatula Jemalong and the superembryogenic line 2HA after two weeks of tissue culture on 10 ÂµM 1-naphthaleneacetic acid (NAA) and 4 ÂµM 6-benzylaminopurine (BAP) (P4 10:4) Experiment Overall Design: Leaf explants from the two genotypes were treated with 10:4 for two weeks; total RNA was extracted and used for hybridization to Affymetrix arrays

2008-06-16 | E-GEOD-8131 | biostudies-arrayexpress

Vegetative and reproductive leaves - 12plex-medicago-2012-06_GENOPEA3.2

Project description:12plex_medicago-2012-06 - vegetative and reproductive leaves - Transcriptome in leaves of Medicago truncatula plants during the remobilization process . Effect of a nitrogen deficiency on this process.Note that lower leaves correspond to vegetative leaves (FV) and upper leaves correspond to leaves of the reproductive part (FR). - Analysis of expression in Medicago truncatula reproductive leaves in untreated and nitrogen deficient plants during remobilization process between beginning of flowering, pod filling and the end of pod filling.

2019-01-01 | GSE109521 | GEO

Cell- and tissue-specific transcriptome analyses of Medicago truncatula root nodules

Project description:We used laser-capture microdissection (LCM) to isolate specific cells from the Medicago truncatula nodule meristem (M), the distal infection (DIZ), the proximal infection zone (PIZ), infected cells (IC) and uninfected cells (UIC) from the fixation zone. Based on Medicago GeneChips, we identified the cell- and tissue-specific programm of gene expression in Medicago truncatula root nodules.

2013-05-29 | GSE43354 | GEO

Effects of Gongronella sp. w5 on Medicago truncatula transcriptome

Project description:To investigate the gene expression levels of Medicago truncatula roots after beneficial fungi Gongronella sp. w5 inoculated.Gongronella sp. w5 promoted M. truncatula growth and caused the accumulation of sucrose in M. truncatula root tissue at 16 day-post-inoculation (dpi) without invading into the root cells. The transport of photosynthetic product sucrose to the rhizosphere by M. truncatula root cells was accelerated by upregulating the SWEET gene.

2020-08-11 | GSE155951 | GEO

microRNA profiling of root tissues and root forming explant cultures in Medicago truncatula

Project description:To identify key miRNAs involved in root meristem formation in M. truncatula, deep sequencing was used to compare the miRNA populations dreived from four tissues. These were; root tip tissue, containing the root apical meristem, elongation zone tissue, root forming callus tissue and non-root forming callus tissue. We identified 83 previously reported miRNAs, 24 new to M. truncatula, in 44 families. For the first time in M. truncatula, members of conserved miRNA families mir165, miR181 and miR397 were found. Bioinformatic analysis identified 38 potential novel miRNAs. Many miRNAs were differentially expressed between tissues, particularly RFC and NRFC. Examination and comparison of the microRNA population of four Medicago truncatula tissue types

2013-04-03 | E-GEOD-45726 | biostudies-arrayexpress

Transcription profiling of root tips from Medicago truncatula Jemalong A17 and TN1.11 after salt stress

Project description:Effect of Salt Stress on root tip transcriptome of Medicago truncatula Jemalong A17 and Medicago truncatula TN1.11

2010-01-01 | E-MEXP-1255 | biostudies-arrayexpress

Transcript profiling of hairy root lines overexpressing MtPAR

Project description:Hairy root lines over-expressing MtPAR using a 35S promoter compared with hairy lines over-expressing GUS gene. Hairy roots were generated in vitro using leaf explants from Medicago A17. The goal of this experiment is to prove that ectopic expression expression of MtPAR is sufficient to induce tannin biosynthesis

2012-01-31 | E-MEXP-3485 | biostudies-arrayexpress

Identification of genes involved in the establishment of plant-microorganism interactions

Project description:affy_pathogen_medicago - In compatible interaction between plants and biotrophic microorganisms, neoformation of organs occurs to ensure an efficient relationship between both partners. During the interaction between Medicago truncatula and Sinorhizobium meliloti, bacteria induce the development of root nodule with a permanent meristem, and chronically infect plant cells from zone II before differentiating into atmospheric nitrogen fixing bacteroids. M. truncatula is also plant host for root-knot nematodes, such as Meloidogyne incognita. During this compatible pathogenic interaction, root-knot nematodes induce redifferenciation of root cells from the vascular cylinder into specialized feeding cells called “giant cells”. Hyperplasia and hypertrophy of the surrounding cells lead to the formation of typical root galls. This phenomenon invokes host pathways in common with those necessary for nitrogen-nodule formation, suggesting that nematode and rhizobia exploit a relative common strategy of plant cell infection at the cellular and molecular levels. In order to highlight key genes involved in gall and nodule developments, parallel laser microdissection of giant cells from galls and cells from zone II of nodules, followed by transcriptomic analysis, were performed. The RNA pools were extracted from these cells, amplified and used for transcriptomic studies with M. truncatula Affymetrix DNA chips. Keywords: organ comparison 12 arrays - Medicago infected with M. incognita or S. meliloti.

2012-03-01 | E-GEOD-19872 | biostudies-arrayexpress

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