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TiO2 nanoparticles' library toxicity (UV and non-UV exposure) – High-throughput in vivo transcriptomics reveals mechanisms

ABSTRACT: Ecotoxicological studies with NPs are nearly all focused on testing one or few NPs. A more systematic approach to compare effects across many NMs with identified different but related characters/descriptors (e.g. different aspect-ratios or surface activities) are only sparsely reported especially in ecotoxicology. There is an urgent need to provide tools that allow us to fast develop an (eco)toxicological dataset containing comprehensive toxicity information relating to a diverse spectra of NPs characters, thus allowing the extrapolation of acquired information across NMs. NM libraries are an excellent tool for alternative testing and modeling. In the present study a custom-designed Fe doped TiO2 NM library was used covering of a wide spectrum of properties. Enchytraeus crypticus (Oligochateta) was used as test species since it is both environmentally relevant (as an important soil representative oligochaete and is also a standard species in soil ecotoxicology studies) and also a genomic model, with a high-throughput (HTP) genomic library. Effects were assessed at the organism (survival, reproduction) and gene level (differential gene expression, microarray).

ORGANISM(S): Enchytraeus crypticus

PROVIDER: GSE81694 | GEO | 2023/03/02

REPOSITORIES: GEO

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