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A. nidulans wild type and atmA null mutant strains transcriptome analysis for polar growth upon HU-release

ABSTRACT: ATM is a phosphatidyl-3-kinase-related protein kinase that functions as a central regulator of the DNA damage response in eukaryotic cells. In humans, mutations in ATM cause the devastating neurodegenerative disease Ataxia Telangiectasia. Previously, we characterized the homologue of ATM (AtmA) in the filamentous fungus Aspergillus nidulans. In addition to its expected role in the DNA damage response, we found that AtmA is also required for polarized hyphal growth. In order to investigate which pathways are controlled by AtmA during proliferation and polar growth, we determined the transcriptional profile of A. nidulans wild type and delta atmA mutant strains in different growth conditions. Conidia from both wild type and delta atmA mutant were incubated with 50 mM of hydroxyurea to synchronize the germlings in the S-phase of cell cycle. After this time, the cultures were centrifuged, washed and pre-warmed drug-free fresh media was aseptically added to the cultures. They were allowed to grow for additional 60, 90 and 120 minutes after the HU-release. At each time point, the germlings were also harvested by centrifugation and used for competitive microarray hybridizations. Our results indicate several genes that have decreased mRNA expression in the delta atmA mutant which are involved in the formation of a polarized hyphae and control of polar growth; in the synthesis of phosphatidic acid; in the ergosterol biosynthesis; and intracellular trafficking, secretion, and vesicular transport. Keywords: gene expression array-based (log2 ratio)

ORGANISM(S): Aspergillus nidulans

PROVIDER: GSE8181 | GEO | 2007/07/25

SECONDARY ACCESSION(S): PRJNA105423

REPOSITORIES: GEO

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A. nidulans wild type and atmA null mutant strains transcriptome analysis in conidia grown for 60, 90 and 120 minutes

Project description:ATM is a phosphatidyl-3-kinase-related protein kinase that functions as a central regulator of the DNA damage response in eukaryotic cells. In humans, mutations in ATM cause the devastating neurodegenerative disease Ataxia Telangiectasia. Previously, we characterized the homologue of ATM (AtmA) in the filamentous fungus Aspergillus nidulans. In addition to its expected role in the DNA damage response, we found that AtmA is also required for polarized hyphal growth. In order to investigate which pathways are controlled by AtmA during proliferation and polar growth, we determined the transcriptional profile of A. nidulans wild type and delta atmA mutant strains in different growth conditions. Conidia from both wild type and delta atmA mutant were incubated with 50 mM of hydroxyurea to synchronize the germlings in the S-phase of cell cycle. After this time, the cultures were centrifuged, washed and pre-warmed drug-free fresh media was aseptically added to the cultures. They were allowed to grow for additional 60, 90 and 120 minutes after the HU-release. At each time point, the germlings were also harvested by centrifugation and used for competitive microarray hybridizations. Our results indicate several genes that have decreased mRNA expression in the delta atmA mutant which are involved in the formation of a polarized hyphae and control of polar growth; in the synthesis of phosphatidic acid; in the ergosterol biosynthesis; and intracellular trafficking, secretion, and vesicular transport. Keywords: gene expression array-based, log2 ratio

2007-07-25 | GSE8183 | GEO

Aspergillus nidulans: Control (0 h starvation) vs. induction of starvation (12 h and 24 h) for WT, delta xprG and delta atmA

Project description:Transcriptional profiling of A. nidulans comparing starvation for 0 (reference), 12 and 24 h. The main objective was to identify genes specifically regulated during starvation by atmA and xprG. The results of the experiment were further validated by real-time PCR. Experimental procedure: Three A. nidulans strains were used in this study: WT, delta atmA and delta xprG. Strains were grown on minimal medium for 24 h (0 h starvation reference), then exposed to 12 and 24 h starvation. atmA: ATM, Ataxia-Telangiectasia mutated; Malavazi, I., Savoldi, M., Da Silva Ferreira, M. E., Soriani, F. M., Bonato, P. S., De Souza Goldman, M. H. and Goldman, G. H. (2007), Transcriptome analysis of the Aspergillus nidulans AtmA (ATM, Ataxia-Telangiectasia mutated) null mutant. Molecular Microbiology, 66: 74-99 (PMID 17880424). xprG: extracellular protease; Margaret E. Katz, Karen-Ann Gray, Brian F. Cheetham, (2006) The Aspergillus nidulans xprG (phoG) gene encodes a putative transcriptional activator involved in the response to nutrient limitation, Fungal Genetics and Biology, 43, 190-199 (PMID 16464624).

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A. fumigatus wild type and delta phoB(PHO80) strains transcriptome analysis for growth upon minimal medium 11 mM Pi

Project description:Phosphate is an essential ion for fungal growth, required for proper DNA and phospholipids biosyntesis, energetic metabolism and signal transduction. The systems for inorganic phosphate (Pi) acquisition in eukaryotic cells (PHO) have been characterized as a low-affinity (that assures a supply of Pi at normal or high external Pi concentrations) and a high-affinity (activated in response to Pi starvation). Here, as an initial step to understand the PHO pathway in A. fumigatus, we characterized the PHO80 homologue, phoB(PHO80). We showed that the delta phoB(PHO80) mutant has a severe polar growth defect and there is an interaction between PhoB(PHO80), calcineurin and calcium metabolism. Microarray hybridizations carried out with RNA obtained from wild type and delta phoB(PHO80) mutant cells showed that Afu4g03610 [phoD(PHO84)], Afu7g06350 [phoE9(PHO89)], Afu4g06020 [phoCPHO81)], and Afu2g09040 (vacuolar transporter Vtc4) were more expressed either in the delta phoB(PHO80) mutant background or in 11 mM Pi. Keywords: gene expression array-based (log2 ratio)

2007-11-09 | GSE9550 | GEO

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Project description:Phosphate is an essential ion for fungal growth, required for proper DNA and phospholipids biosyntesis, energetic metabolism and signal transduction. The systems for inorganic phosphate (Pi) acquisition in eukaryotic cells (PHO) have been characterized as a low-affinity (that assures a supply of Pi at normal or high external Pi concentrations) and a high-affinity (activated in response to Pi starvation). Here, as an initial step to understand the PHO pathway in A. fumigatus, we characterized the PHO80 homologue, phoB(PHO80). We showed that the delta phoB(PHO80) mutant has a severe polar growth defect and there is an interaction between PhoB(PHO80), calcineurin and calcium metabolism. Microarray hybridizations carried out with RNA obtained from wild type and delta phoB(PHO80) mutant cells showed that Afu4g03610 [phoD(PHO84)], Afu7g06350 [phoE9(PHO89)], Afu4g06020 [phoCPHO81)], and Afu2g09040 (vacuolar transporter Vtc4) were more expressed either in the delta phoB(PHO80) mutant background or in 0.1 mM Pi. Keywords: gene expression array-based (log2 ratio)

2007-11-09 | GSE9548 | GEO

Drosophila melanogaster selection lines confronted with Aspergillus nidulans

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2020-05-09 | E-MTAB-9011 | biostudies-arrayexpress

The COP9 signalosome mediates transcriptional and metabolic response during fungal development

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2010-09-09 | E-GEOD-22442 | biostudies-arrayexpress

Aspergillus nidulans

Project description:A. nidulans wild type and atmA null mutant strains transcriptome analysis for polar growth upon HU-release

| PRJNA105423 | ENA

Rsd timecourse

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