Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Position effects influence HIV provirus latency reversal

ABSTRACT: We propose a technology B-HIVE, which allows us to map HIV integrations in a cell population and measure their individual transcription. The principle of B-HIVE is to tag individual viral genomes with a unique barcode of 20 nucleotides that allows us to track the viral transcripts produced by each provirus in the cell population.

ORGANISM(S): Homo sapiens

PROVIDER: GSE82061 | GEO | 2016/09/17

SECONDARY ACCESSION(S): PRJNA323993

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The chromatin landscape at the HIV-1 provirus integration site determines viral expression

Project description:We investigated the link between HIV-1 integration by using B-HIVE and LEDGINs. B-HIVE tracks insert-specific HIV expression by tagging a unique barcode in the HIV genome. LEDGINs are antivirals that inhibit the interaction between HIV-IN and its chromatin tethering cofactor LEDGF/p75. They are known to retarget HIV-1 integration sites. Here, we confirmed that LEDGIN treatment retargets integration out of transcriptionally active regions and reduce HIV expression. Silent provirus was located at increased distance to H3K36me3, the recognition marker of LEDGF/p75, after treatment with LEDGINs. Viral RNA expression was also influenced by the proximity of enhancers, regardless of the presence of LEDGINs.

2020-07-03 | GSE135295 | GEO

Chromatin maturation of the HIV provirus in primary resting CD4+ T cells

Project description:Human immunodeficiency virus (HIV) infection is a chronic condition, where viral DNA integrates into the genome. The fate of the provirus determines the infection course. Latently infected cells form a persistent, heterogeneous reservoir. The reservoir that reinstates an active infection comprises cells with intact provirus that can be reactivated. We confirmed that latent cells from patients exhibited active transcription throughout the provirus. To find transcriptional determinants, we characterized the establishment and maintenance of latency during proviral chromatin maturation in primary CD4+ T-cells for four months after HIV infection. As heterochromatin (marked with H3K9me3 or H3K27me3) gradually stabilized, the provirus became less accessible and lost activation potential. In a subset of infected cells, active marks (i.e., H3K27ac) remained detectable, even after prolonged proviral silencing. After T-cell activation, the proviral activation occurred uniquely in cells with H3K27ac-marked proviruses. Our observations suggested that, after transient proviral activation, cells were actively returned to latency.

2019-12-16 | GSE121055 | GEO

The chromatin landscape at the HIV-1 provirus integration site determines viral expression

Project description:The chromatin landscape at the HIV-1 provirus integration site determines viral expression

| PRJNA558259 | ENA

Systematic Identification of Factors Crucial for Provirus Silencing in Embryonic Stem Cells (ChIP-seq)

Project description:Our study reports the first genome-wide atlas of functional nodes that mediate proviral silencing in ESCs. It provides evidences for the comprehensive, interconnected and multi-layered genetic/epigenetic machineries by which ESCs maintain the repressive state of provirus and ERVs. ChIP-seq analysis of Chaf1a, Trim28, Sumo2 and Zfp809 to demonstrate the mechanism of the silencing of Endogenous retroviruses

2015-09-14 | E-GEOD-70799 | biostudies-arrayexpress

Systematic Identification of Factors Crucial for Provirus Silencing in Embryonic Stem Cells (RNA-seq)

2015-09-14 | GSE70863 | GEO

Systematic Identification of Factors Crucial for Provirus Silencing in Embryonic Stem Cells (ChIP-seq)

2015-09-14 | GSE70799 | GEO

Systematic Identification of Factors Crucial for Provirus Silencing in Embryonic Stem Cells (RNA-seq)

Project description:Our study reports the first genome-wide atlas of functional nodes that mediate proviral silencing in ESCs. It provides evidences for the comprehensive, interconnected and multi-layered genetic/epigenetic machineries by which ESCs maintain the repressive state of provirus and ERVs. RNA-seq analysis to determine the regulated endogenous retroviruses by Histone chaperones (Chaf1a/b), sumoylation factors (Sumo2/ Ube2i/Sae1/Uba2/Senp6) and chromatin modifiers (Trim28/Eset/Atf7ip)

2015-09-14 | E-GEOD-70863 | biostudies-arrayexpress

Haloferax volcanii provirus-free strain transcriptome analysis

Project description:Transcriptome analysis of Haloferax volcanii provirus-free strain vs wild-type H100

| PRJEB83039 | ENA

Transcription of HIV-1 at sites of intact latent provirus integration

Project description:Transcription of HIV-1 at sites of intact latent provirus integration

| PRJNA1114035 | ENA

Human parvovirus

Project description:Human parvovirus viral integrations into human cell types

| PRJNA208398 | ENA