Genome-wide identification of nitric-oxide regulated H3K9/14ac sites in Arabidopsis thaliana
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ABSTRACT: We report on the identification of nitric-oxide regulated H3K9/14ac sites in Arabidopsis. For this purpose liquid grown Arabidopsis seedlings were treated with the natural NO-donor S-nitrosoglutathione (GSNO), GSNO in combination with the NO-scavenger cPTIO, GSH (control) or TSA (positive control). Material was crosslinked and harvested 3h and 16h after onset of the treatments. Then, quantitative analysis of the H3K9/14ac patterns across these treatment was performed using DiffBind. By comparison of the GSNO and GSNO/cPTIO treatment, NO-regulated H3K9/14ac sites were identified. These sites were then annotated to the nearest TSS. GO enrichment analysis revealed that NO affects H3K9/14ac at many stress-responsive and photosynthetic genes.
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE82075 | GEO | 2017/03/07
SECONDARY ACCESSION(S): PRJNA323929
REPOSITORIES: GEO
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